Characterization of isoform expression and subcellular distribution of MYPT1 in intestinal epithelial cells
Characterization of isoform expression and subcellular distribution of MYPT1 in intestinal epithelial cells
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肠上皮细胞中 MYPT1 异构体表达和亚细胞分布的表征
DOI：
10.1016/j.gene.2016.04.048
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发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
He Wei-Qi
中科院分区：
文献类型：
--
作者：
Zha Juan-Min;Li Hua-Shan;Wang Yi-Tang;Lin Qian;Tao Min;He Wei-Qi
肠上皮通透性的调节需要肌球蛋白调节轻链(MLC)的磷酸化。MLC的磷酸化状态受肌球蛋白轻链磷酸酶(MLCP)活性的调节。MLCP的催化亚基(PP1c-δ)的活性依赖于其调节亚基(MYPT1)。在这项研究中，我们发现在人类肠上皮细胞(Caco-2)和分离的小鼠肠上皮细胞(IECs)中存在两种MYPT1亚型，全长和变体2。在融合的Caco-2细胞中，MYPT1分布于细胞-细胞接触处，并与F-肌动蛋白共定位。这些结果表明，MYPT1亚型在肠上皮细胞中表达，MYPT1可能参与了肠上皮屏障功能的调节。
The regulation of intestinal epithelial permeability requires phosphorylation of myosin regulatory light chain (MLC). The phosphorylation status of MLC is regulated by myosin light chain phosphatase (MLCP) activities. The activity of the catalytic subunit of MLCP (PP1cδ) toward MLC depends on its regulatory subunit (MYPT1). In this study, we revealed the presence of two MYPT1 isoforms, full length and variant 2 in human intestinal (Caco-2) epithelial cells and isolated intestinal epithelial cells (IECs) from mice. In confluent Caco-2 cells, MYPT1 was distributed at cell–cell contacts and colocalized with F-actin. These results suggest that MYPT1 isoforms are expressed in intestinal epithelial cells and MYPT1 may be involved in the regulation of intestinal epithelial barrier function.