IMMUNOCHEMICAL IDENTIFICATION OF VIRAL AND NON-VIRAL PROTEINS OF THE RESPIRATORY SYNCYTIAL VIRUS VIRION
IMMUNOCHEMICAL IDENTIFICATION OF VIRAL AND NON-VIRAL PROTEINS OF THE RESPIRATORY SYNCYTIAL VIRUS VIRION
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DOI：
10.1128/iai.37.1.243-249.1982
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
GERIN, JL
中科院分区：
文献类型：
--
作者：
FERNIE, BF;GERIN, JL
用免疫化学方法鉴定了13种与传染性呼吸道合胞病毒病毒粒子相关的多肽。这些多肽中的八个（VP 200、VP 84、VP 66、VP 43、VP 40、VP 37、VP 28和VP 19）被鉴定为病毒特异性的。另外两种多肽（VP）22和（VP）12暂时被认为是病毒来源。三种非病毒蛋白质也与感染性病毒粒子密切相关。这些非病毒蛋白被鉴定为细胞肌动蛋白和2个具有牛血清白蛋白免疫特异性的蛋白。VP 40被鉴定为主要的核糖核蛋白。基于与副粘病毒蛋白的生物化学和生物物理学相似性，认为其他呼吸道合胞病毒蛋白具有这些特定的病毒功能：VP 84，血凝素; VP 66，未解离的融合蛋白，F1，2; VP 43，F1;和VP 19，F2。VP 66含有参与病毒感染性的主要决定因素，因为所有测试的中和抗体，包括单克隆抗体，都沉淀这种蛋白质。
Thirteen polypeptides associated with the infectious respiratory syncytial virus virion were identified by immunochemical methods. Eight of these polypeptides (VP200, VP84, VP66, VP43, VP40, VP37, VP28 and VP19) were identified as virus specific. Two other polypeptides, (VP) 22 and (VP) 12, are provisionally considered to be of viral origin. Three nonviral proteins are also intimately associated with the infectious virion. These nonviral proteins were identified as cellular actin and 2 proteins with bovine serum albumin immunospecificity. VP40 was identified as the major ribonucleoprotein. Based on biochemical and biophysical similarities with paramyxovirus proteins, other respiratory syncytial virus proteins are believed to have these specific viral functions: VP84, hemagglutinin; VP66, undissociated fusion protein, F1,2; VP43, F1; and VP19, F2. VP66 contains a major determinant involved in viral infectivity since all neutralizing antibodies tested, including a monoclone, precipitated this protein.