Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects
Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects
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DOI：
10.1080/13813455.2017.1369547
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发表时间：
2018-01-01
影响因子：
3
通讯作者：
Jensen, Jorgen
中科院分区：
文献类型：
--
作者：
Lund, Jenny;Tangen, Daniel S.;Jensen, Jorgen
我们假设健康年轻人的骨骼肌在氧化能力和纤维类型组成上有很大的差异，因此我们的目的是研究健康男性不同程度最大摄氧量的股外侧肌活检和肌管中的葡萄糖代谢。经过训练和中间训练的受试者在体内表现出更高的碳水化合物氧化能力。活检组织和肌管中的纤维类型分布在不同组之间没有差异。活检组织中I型纤维的表达与肌管之间没有相关性。训练后的肌管具有较高的脱氧葡萄糖积累量和葡萄糖氧化分数(葡萄糖氧化相对于葡萄糖摄取)，并且对急性添加的油酸对细胞的抑制作用更敏感。尽管骨骼肌活检组织和培养细胞之间缺乏纤维类型的相关性，但训练有素的受试者的肌管在增强葡萄糖代谢和代谢灵活性方面保留了它们在体外的一些表型。
We hypothesised that skeletal muscles of healthy young people have a large variation in oxidative capacity and fibre-type composition, and aimed therefore to investigate glucose metabolism in biopsies and myotubes isolated from musculus vastus lateralis from healthy males with varying degrees of maximal oxygen uptake. Trained and intermediary trained subjects showed higher carbohydrate oxidation in vivo. Fibre-type distribution in biopsies and myotubes did not differ between groups. There was no correlation between fibre-type I expression in biopsies and myotubes. Myotubes from trained had higher deoxyglucose accumulation and fractional glucose oxidation (glucose oxidation relative to glucose uptake), and were also more sensitive to the suppressive action of acutely added oleic acid to the cells. Despite lack of correlation of fibre types between skeletal muscle biopsies and cultured cells, myotubes from trained subjects retained some of their phenotypes in vitro with respect to enhanced glucose metabolism and metabolic flexibility.