Transcriptome analyses reveal the utilization of nitrogen sources and related metabolic mechanisms of Sporosarcina pasteurii.
Transcriptome analyses reveal the utilization of nitrogen sources and related metabolic mechanisms of Sporosarcina pasteurii.
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DOI：
10.1371/journal.pone.0246818
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Hu B
中科院分区：
文献类型：
--
作者：
Pei D;Liu Z;Wu W;Hu B
近年来，巴斯德孢子八菌（S.pasteurii）已成为微生物诱导碳酸钙沉淀（MICP）中最受欢迎的细菌之一。基于可以诱导碳酸钙沉淀的高效脲酶，已经开发了各种应用。然而，与巴氏沙门氏菌生物矿化相关的代谢机制尚未明确阐明。细菌培养和生物矿化过程消耗大量尿素或铵盐，这些尿素或铵盐通常用作农业肥料，更不用说过度使用这些原料可能造成的环境污染。因此，迫切需要揭示巴氏酵母的氮利用和代谢机制。在本文中，我们通过转录组分析比较了三种不同培养条件下巴氏沙门氏菌的生长和基因表达。 GO和KEGG分析表明，铵和尿素都是巴斯德沙门氏菌的直接氮源，在没有铵或尿素的情况下，细菌无法正常生长。据我们所知，该论文是第一篇通过转录组方法揭示巴氏酵母氮利用机制的论文。此外，铵的存在可能促进细胞内 ATP 的合成并增强细菌的运动性。巴氏沙门氏菌中应该存在与脲酶催化的尿素水解相关的 ATP 合成机制。
In recent years, Sporosarcina pasteurii (S. pasteurii) has become one of the most popular bacteria in microbially induced calcium carbonate precipitation (MICP). Various applications have been developed based on the efficient urease that can induce the precipitation of calcium carbonate. However, the metabolic mechanism related to biomineralization of S. pasteurii has not been clearly elucidated. The process of bacterial culture and biomineralization consumes a large amount of urea or ammonium salts, which are usually used as agricultural fertilizers, not to mention probable environmental pollutions caused by the excessive use of these raw materials. Therefore, it is urgent to reveal the mechanism of nitrogen utilization and metabolism of S. pasteurii. In this paper, we compared the growth and gene expression of S. pasteurii under three different culture conditions through transcriptome analyses. GO and KEGG analyses revealed that both ammonium and urea were direct nitrogen sources of S. pasteurii, and the bacteria could not grow normally in the absence of ammonium or urea. To the best of our knowledge, this paper is the first one to reveal the nitrogen utilization mechanism of S. pasteurii through transcriptome methods. Furthermore, the presence of ammonium might promote the synthesis of intracellular ATP and enhance the motility of the bacteria. There should be an ATP synthesis mechanism associated with urea hydrolysis catalyzed by urease in S. pasteurii.
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