Efficient fine mapping of the naked caryopsis gene (nud) by HEGS (High Efficiency Genome Scanning)/AFLP in barley
Efficient fine mapping of the naked caryopsis gene (nud) by HEGS (High Efficiency Genome Scanning)/AFLP in barley
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DOI：
10.1007/s00122-003-1413-y
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发表时间：
2003-12-01
影响因子：
5.4
通讯作者：
Kawasaki, S
中科院分区：
文献类型：
--
作者：
Kikuchi, S;Taketa, S;Kawasaki, S
大麦（Hordeum vulgare L.）的剥壳或裸颖果性状是其可食性和驯化过程的重要性状。控制裸颖果性状的单隐性基因nud位于7H染色体长臂上。为了有效地绘制nud位点周围的精细图谱，将HEGS （High Efficiency Genome Scanning）电泳系统与扩增片段长度多态性（AFLP）相结合。从1894个引物组合的散装分离分析中，选择12个AFLP片段作为连锁标记。在作图时，使用了裸型Kobinkatagi和壳型Triumph杂交的151个F-2种群。7个AFLP标记定位在nud区附近。绘制了一张精细的地图，分辨率比以前提高了一个数量级，并附有七个锚点标记。在7个AFLP连锁标记（KT1-7）中，KT1、KT2和KT6共显性，用HEGS非变性凝胶电泳后，检测到KT1、KT2和KT6在相同长度片段中存在单核苷酸多态性（snp）。nud位点与KT3和KT7共分离，位于KT2和KT4的两侧，分别位于近端0.3 cm和远端1.2 cm。其中4个AFLP标记转化为序列特征扩增区（SCAR）标记，其中1个是与nud基因共分离的显性标记。
The hulled or naked caryopsis character of barley (Hordeum vulgare L.) is an important trait for edibility and to follow its domestication process. A single recessive gene, nud, controls the naked caryopsis character, and is located on the long arm of chromosome 7H. To develop a fine map around the nud locus efficiently, the HEGS (High Efficiency Genome Scanning) electrophoresis system was combined with amplified fragment length polymorphism (AFLP). From bulked segregant analysis of 1,894 primer combinations, 12 AFLP fragments were selected as linked markers. For mapping, an F-2 population of 151 individuals derived from a cross between Kobinkatagi (naked type) and Triumph (hulled type) was used. Seven AFLP markers were localized near the nud region. A fine map was developed with one-order higher resolution than before, along with the seven anchor markers. Among the seven linked AFLP markers (KT1-7), KT1, KT2 and KT6 were co-dominant, and the former two were detected for their single-nucleotide polymorphisms (SNPs) in the same length of fragments after electrophoresis with the non-denaturing gels of HEGS. The nud locus has co-segregated with KT3 and KT7, and was flanked by KT2 and KT4, at the 0.3-cM proximal and the 1.2-cM distal side, respectively. Four of these AFLP markers were converted into sequence-characterized amplified region (SCAR) markers, one of which was a dominant marker co-segregating with the nud gene.