PRODUCTIVE AND ABORTIVE INITIATION OF TRANSCRIPTION INVITRO AT THE LAC UV5 PROMOTER
PRODUCTIVE AND ABORTIVE INITIATION OF TRANSCRIPTION INVITRO AT THE LAC UV5 PROMOTER
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DOI：
10.1021/bi00566a031
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发表时间：
1980-01-01
期刊：
影响因子：
2.9
通讯作者：
STEFANO, JE
中科院分区：
文献类型：
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作者：
GRALLA, JD;CARPOUSIS, AJ;STEFANO, JE
测定了Lac UV5启动子[EscherichiaColi]体外转录的产生率和流产起始率，并与噬菌体lambda的测定值进行了比较。和T7启动子。在低到中等浓度的启动三磷酸腺苷的范围内，有效启动乳糖转录本的速率常数始终较低。在低到中等浓度的三磷酸腺苷中，紫胶二核苷四磷酸的流产起始也较慢。这些数据表明，启动子之间的起始率存在显著差异。这些差异可能是启动子特异性转录启动过程中RNA聚合酶循环的初始mRNA序列和程度的结果。
The rates of productive and abortive initiation of transcription in vitro at the lac UV5 promoter [Escherichia coli] were determined and compared to values determined for phage .lambda. and T7 promoters. The rate constants for productive initiation of lac transcript are consistently lower over a range of low to moderate concentration of initiating ATP. Abortive initiation of lac dinucleoside tetraphosphate is also slower at low to moderate concentrations of ATP. These data demonstrate the existence of significant differences in initiation rate among promoters. These differences may be a consequence of the initial mRNA sequences and extents of RNA polymerase cycling during initiation of promoter-specific transcription.