Phospholipid scramblase 1 mediates hepatitis C virus entry into host cells
Phospholipid scramblase 1 mediates hepatitis C virus entry into host cells
复制标题
磷脂扰乱酶1介导丙型肝炎病毒进入宿主细胞
DOI：
10.1016/j.febslet.2011.07.019
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发表时间：
2011-09-02
期刊：
影响因子：
3.5
通讯作者：
Wang, Jian
中科院分区：
文献类型：
--
作者：
Gong, Qiaoling;Cheng, Min;Wang, Jian
Hepatitis C virus (HCV) infects human hepatocytes through several host factors. However, other prerequisite factors for viral entry remain to be identified. Using a yeast two-hybrid screen, we found that human phospholipid scramblase 1 interacts with HCV envelope proteins E1 and E2. These physical interactions were confirmed by co-immunoprecipitation and GST pull-down assays. Knocking down the expression of PLSCR1 inhibited the entry of HCV pseudoparticles. Moreover, PLSCR1 was required for the initial attachment of HCV onto hepatoma cells, where it specifically interacted with entry factor OCLN. We show that PLSCR1 is a novel attachment factor for HCV entry.Structured summary of protein interactions:OCLN physically interacts with PLSCR1 by anti tag coimmunoprecipitation (View interaction)E2 physically interacts with PLSCR1 by anti tag coimmunoprecipitation (View interaction)PLSCR1 physically interacts with E2 by anti tag coimmunoprecipitation (View interaction)PLSCR1 physically interacts with OCLN by anti tag coimmunoprecipitation (View interaction)PLSCR1 physically interacts with E2 by pull down (View interaction)E1 physically interacts with PLSCR1 by two hybrid (View interaction)E2 physically interacts with PLSCR1 by two hybrid (View interaction)PLSCR1 physically interacts with E1 by anti tag coimmunoprecipitation (View interaction)E2 physically interacts with PLSCR1 by pull down (View interaction)E1 physically interacts with PLSCR1 by pull down (View interaction) (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
