Muscarinic‐Activated K+ Current in Bovine Aortic Endothelial Cells
Muscarinic‐Activated K+ Current in Bovine Aortic Endothelial Cells
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DOI：
10.1161/01.res.62.6.1059
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发表时间：
1988-06
影响因子：
20.1
通讯作者：
S. Olesen;P. Davies;D. Clapham
中科院分区：
文献类型：
--
作者：
S. Olesen;P. Davies;D. Clapham
分离培养的动脉内皮细胞表达乙酰胆碱（ACh）激活的K+电流，除了内向整流电流，其电导是不受ACh。胆碱能K+电流被阿托品（1 μM）特异性阻断，并显示ACh的单一饱和动力学（半数最大刺激51 nM ACh）。与心脏毒蕈碱受体门控K+通道不同，其刺激似乎不依赖于百日咳毒素敏感的GTP结合蛋白。内皮细胞毒蕈碱K+电流的激活导致超极化可能代表ACh血管舒张作用的初始成分。
Isolated, cultured arterial endothelial cells express an acetylcholine (ACh)-activated K+ current in addition to an inward rectifier current whose conductance is unaffected by ACh. The cholinergic K+current is specifically blocked by atropine (1 μM) and shows single saturation kinetics with ACh (half-maximal stimulation 51 nM ACh). Unlike the cardiac muscarinic receptor-gated K+ channel, its stimulation appears independent of a pertussis toxin—sensitive GTP-binding protein. Activation of the endothelial muscarinic K+ current resulting in hyperpolarization may represent an initial component of the vasodilatory effect of ACh.