TNF-alpha induces interleukin-8 and endothelin-1 expression in human endothelial cells with different redox pathways.
TNF-alpha induces interleukin-8 and endothelin-1 expression in human endothelial cells with different redox pathways.
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TNF-α 通过不同的氧化还原途径诱导人内皮细胞中白细胞介素 8 和内皮素 1 的表达。
DOI：
10.1016/j.bbrc.2004.12.109
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Chen,Changyi
中科院分区：
文献类型：
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作者：
Zhao,Ruo-Zhi;Chen,Xilin;Yao,Qizhi;Chen,Changyi
我们研究了TNF-α对白细胞介素-8 （IL-8）和内皮素-1 （ET-1）表达的影响及其不同的信号转导途径。用TNF-α处理人真皮微血管内皮细胞（HMECs）。Northern blot分析，TNF-α浓度为50、100、200和400U/ml时，与对照组相比，IL-8 mRNA的表达分别显著增加206%、252%、211%和158% （p<0.05）。通过腺病毒介导的基因转移或添加外源过氧化氢（H2O2）过表达人超氧化物歧化酶（SOD）可显著增强TNF-α-诱导的IL-8 mRNA表达。50、100和200U/ml TNF-α处理HMECs后，ET-1 mRNA表达量分别显著增加71%、82%和66% （p<0.05）。SOD基因转移和外源h2o2可显著抑制TNF-α-诱导的ET-1 mRNA表达。因此，TNF-α可能通过不同的氧化还原信号通路显著诱导hmec中IL-8和ET-1基因的表达。h2o2增强TNF-α-诱导的IL-8表达，抑制TNF-α-诱导的ET-1表达。
We investigated the effect of TNF-α on interleukin-8 (IL-8) and endothelin-1 (ET-1) expression, and their different signal transduction pathways. Human dermal microvascular endothelial cells (HMECs) were treated with TNF-α. By Northern blot analysis, TNF-α at 50, 100, 200, and 400U/ml significantly induced IL-8 mRNA expression by 206%, 252%, 211%, and 158%, respectively, as compared to controls (p<0.05). Overexpression of human superoxide dismutase (SOD) by adenovirus-mediated gene transfer or addition of exogenous hydrogen peroxide (H2O2) significantly enhanced TNF-α-induced IL-8 mRNA expression. Furthermore, HMECs treated with TNF-α at 50, 100, and 200U/ml significantly increased ET-1 mRNA expression by 71%, 82%, and 66%, respectively (p<0.05). By contrast, SOD gene transfer and exogenous H2O2significantly inhibited TNF-α-induced ET-1 mRNA expression. Thus, TNF-α significantly induces both IL-8 and ET-1 gene expression in HMECs possibly through different redox signaling pathways. H2O2enhances TNF-α-induced IL-8 expression, but inhibits TNF-α-induced ET-1 expression.