Culture conditions improving the production of jadomycin B
Culture conditions improving the production of jadomycin B
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DOI：
10.1007/s10295-006-0113-4
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发表时间：
2006-09-01
影响因子：
3.4
通讯作者：
Vining, Leo C.
中科院分区：
文献类型：
--
作者：
Jakeman, David L.;Graham, Cathy L.;Vining, Leo C.
雅多霉素是由委内瑞拉链霉菌ISP 5230在热或乙醇休克或噬菌体感染后产生的苯并恶唑菲啶抗生素的独特家族。我们通过改变碳源、缓冲液、接种量和乙醇休克时间来修改培养条件，从而减少生长时间并提高雅多霉素B产量。我们优化的条件使用葡萄糖作为碳源，MOPS作为缓冲液，低浓度的磷酸盐，确定的接种物浓度和立即乙醇休克诱导雅多霉素B生产;结果与以前的研究相反。改变的培养基将有利于分离相关的雅多霉素B同系物。
The jadomycins are a unique family of benzoxazolophenanthridine antibiotics produced by Streptomyces venezuelae ISP5230 following heat or ethanol shock or phage infection. We have modified the culture conditions by altering the carbon source, buffer, inoculum size, and timing of ethanol shock, thereby reducing growing times and improving jadomycin B production. Our optimized conditions use glucose as the carbon source, MOPS as buffer, low concentrations of phosphate, a defined inoculum concentration and an immediate ethanol shock to induce jadomycin B production; results that contrast previous studies. The altered media will facilitate the isolation of related jadomycin B congeners.