Relationship of VH and VL genes encoding three idiotypic families of anti-p-azobenzenearsonate antibodies.
Relationship of VH and VL genes encoding three idiotypic families of anti-p-azobenzenearsonate antibodies.
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编码抗对偶氮苯胂酸抗体的三个独特型家族的 VH 和 VL 基因的关系。
DOI：
10.1073/pnas.83.4.1050
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发表时间：
1986
影响因子：
11.1
通讯作者：
Nisonoff,A
中科院分区：
文献类型：
--
作者：
Robbins,PF;Rosen,EM;Haba,S;Nisonoff,A
We describe here an intrastrain, cross-reactive idiotype (CRI), CRID, associated with anti-p-azobenzenearsonate antibodies of the A/J strain of mouse and distinguishable, by some but not by all of its idiotopes, from the major anti-p-azobenzenearsonate idiotype (CRIA). Molecules carrying the CRID idiotype have heavy chain variable-segment sequences that are identical or nearly identical to that of the germ-line-encoded heavy chain variable sequence of CRIA. Their light chain variable sequences are very similar to those present in a third idiotypic family, CRIC, that is a minor CRI in the A/J strain but a major CRI in BALB/c. This appears to represent a form of combinational diversity, in which the heavy and light chain variable region genes of two unrelated idiotypic families interact to form a third family, all involving antibodies of the same antigen-binding (anti-p-azobenzenearsonate) specificity. The D region of CRID, in the six monoclonal representatives studied (three IgMs, three IgGs), is unusual in that it comprises a single amino acid--arginine or serine; there are eight amino acids in the D region of CRI+A antibodies. Three different heavy chain joining regions, JH1, JH2, and JH3, are utilized. A serological reagent was developed that identifies CRID; it was used to show that the idiotype is present in relatively high concentration, comparable to that of CRIA, in anti-p-azobenzenearsonate antibodies taken soon after primary immunization. In serum taken later CRIA greatly predominates, but CRID persists at low levels.
通过抗原受体和前体细胞分析评估 B 细胞多样化。
DOI：
--
发表时间：
1976
期刊：
Annales d'immunologie
影响因子：
--
作者：
N. Klinman;Allan R. Pickard;Nolan H. Sigal;Patricia J. Gearhart;E. Metcalf;Susan K. Pierce
通讯作者：
Susan K. Pierce
用于固相合成寡脱氧核糖核苷酸的连续流磷酸三酯法。
DOI：
--
发表时间：
1983
期刊：
Cold Spring Harbor Symposia on Quantitative Biology
影响因子：
--
作者：
M. Gait;H. Matthes;M. Singh;B. Sproat;R. Titmas
通讯作者：
R. Titmas