Photic induction of Period gene expression is reduced in Clock mutant mice.
Photic induction of Period gene expression is reduced in Clock mutant mice.
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Clock突变小鼠中Period基因表达的光诱导减少。
DOI：
10.1097/00001756-199902250-00031
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发表时间：
1999
期刊：
影响因子：
1.7
通讯作者：
Weaver,DR
中科院分区：
文献类型：
--
作者：
Shearman,LP;Weaver,DR
时钟突变导致异常的昼夜行为和缺陷的转录活性时钟，基本螺旋-环-螺旋(bHLH)/PAS蛋白。采用原位杂交技术评估Clock突变是否影响小鼠视交叉上核（SCN）中mPer1、mPer2和c-fos的光诱导。夜间将野生型小鼠暴露在15分钟的光脉冲下，快速诱导c-fos mRNA的表达，mPer1和mPer2 mRNA随后达到峰值。光暴露也增加了纯合子Clock突变小鼠SCN中c-fos、mPer1和mPer2 mRNA的水平，但对光的响应幅度明显降低。时钟似乎在昼夜节律光接受中发挥作用，这与它在昼夜节律振荡机制中的作用不同。
The Clock mutation leads to abnormal circadian behavior and defective transcriptional activity of CLOCK, a basic helix-loop-helix (bHLH)/PAS protein. In situ hybridization was used to assess whether the Clock mutation affects the photic induction of mPer1, mPer2, and c-fos in the mouse suprachiasmatic nucleus (SCN). Exposure of wild-type mice to a 15 min light pulse at night rapidly induced expression of c-fos mRNA, with mPer1 and mPer2 mRNAs peaking later. Light exposure also increased c-fos, mPer1 and mPer2 mRNA levels in the SCN of homozygous Clock mutant mice, but the amplitude of the response to light was significantly reduced. Clock appears to play a role in circadian photoreception that is distinct from its role in the circadian oscillatory mechanism.