Bovine intelectins: cDNA sequencing and expression in the bovine intestine
Bovine intelectins: cDNA sequencing and expression in the bovine intestine
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DOI：
10.1016/j.rvsc.2008.06.002
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发表时间：
2009-04-01
影响因子：
2.4
通讯作者：
Pemberton, Alan D.
中科院分区：
文献类型：
--
作者：
Blease, Stacey C.;French, Anne T.;Pemberton, Alan D.
Intelectins (Itlns) 是在先天免疫中具有潜在作用的凝集素，能够通过呋喃半乳糖残基结合细菌。 Itlns 还充当抗菌糖蛋白乳铁蛋白 (Lf) 的肠道受体。由于 Lf 与肠出血性大肠杆菌 O157:H7 (EHEC) 强烈结合，我们的目的是确定 Lf 受体在直肠末端（牛 EHEC 的好发部位）中的表达。我们对两种牛智力素（Itln1 和 Itln2）进行了测序，结果表明两者都在皱胃和直肠中表达，但在空肠中表达量很少。 Itln2 在末端直肠的表达显着高于近端直肠。所有检查的样品中均表达乳铁蛋白。因此，我们已经证明了两种新型牛 Itln，并表明它们与 Lf 一起在胃肠道中表达，在那里它们可能与微生物病原体相互作用。 (c) 2008 Elsevier Ltd. 保留所有权利。
Intelectins (Itlns) are lectins with potential roles in innate immunity, capable of binding bacteria via galactofuranose residues. Itlns also function as intestinal receptors for the antimicrobial glycoprotein lactoferrin (Lf). Since Lf binds strongly to enterohemorrhagic Escherichia coli O157:H7 (EHEC), we aimed to determine the expression of Lf receptor in terminal rectum, the site of predilection of EHEC in cattle. We sequenced two bovine intelectins (Itln1 and Itln2) and showed that both were expressed in abomasum and rectum, but expression appeared minimal in the jejunum. There was significantly higher expression of Itln2 in terminal rather than proximal rectum. Lactoferrin was expressed in all samples examined. Thus, we have demonstrated two novel bovine Itlns and shown that they are expressed along with Lf in the gastrointestinal tract, where they may interact with microbial pathogens. (c) 2008 Elsevier Ltd. All rights reserved.