Clarithromycin Resistance Mechanisms of Epidemic β-Lactamase-Nonproducing Ampicillin-Resistant Haemophilus influenzae Strains in Japan.
Clarithromycin Resistance Mechanisms of Epidemic β-Lactamase-Nonproducing Ampicillin-Resistant Haemophilus influenzae Strains in Japan.
复制标题
日本流行的不产β-内酰胺酶氨苄青霉素耐药流感嗜血杆菌菌株的克拉霉素耐药机制。
DOI：
10.1128/aac.00163-16
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Noguchi N.
中科院分区：
文献类型：
--
作者：
Seyama S;Wajima T;Nakaminami H;Noguchi N.
本研究的目的是阐明不产生氨苄西林的β-内酰胺酶耐药流感嗜血杆菌菌株的克拉霉素耐药机制。在所有耐克拉霉素菌株中，facrb转录物水平显著升高，并且这些菌株的acrr发生移码突变。将该突变引入流感嗜血杆菌Rd后，产生了具有与供体菌株相同MIC的耐克拉霉素转化。我们的研究结果表明，这种突变通过增加facrb的转录而赋予克拉霉素耐药性。
The aim of this study was to clarify the clarithromycin resistance mechanisms of β-lactamase-nonproducing ampicillin-resistant Haemophilus influenzae strains. In all clarithromycin-resistant strains, the transcript level ofacrBwas significantly elevated, and these strains had a frameshift mutation inacrR. Introduction of theacrRmutation into H. influenzae Rd generated a clarithromycin-resistant transformant with the same MIC as the donor strain. Our results indicate that theacrRmutation confers clarithromycin resistance by the increasing the transcription ofacrB.