Responses of gill mitochondria-rich cells in Mozambique tilapia exposed to acidic environments (pH 4.0) in combination with different salinities
Responses of gill mitochondria-rich cells in Mozambique tilapia exposed to acidic environments (pH 4.0) in combination with different salinities
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DOI：
10.1016/j.cbpa.2010.12.003
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发表时间：
2011-04-01
影响因子：
2.3
通讯作者：
Kaneko, Toyoji
中科院分区：
文献类型：
--
作者：
Furukawa, Fumiya;Watanabe, Soichi;Kaneko, Toyoji
在暴露于低渗酸性水时，硬骨鱼遭受血液渗透压和pH值的降低，并因此激活离子调节和酸碱调节机制以恢复被扰乱的离子和酸碱平衡。在莫桑比克罗非鱼Oreochromis mossambicus暴露于酸性（pH 4.0）或中性（pH 7.4-7.7）淡水与0 mM或50 mM NaCl的组合，我们研究了鳃富（MR）细胞的功能和形态变化。我们检测了鳃中Na（+）/H（+）交换体3（NHE 3）、Na（+）/Cl（-）协同转运体（NCC）、液泡型H（+）-ATP酶（V-ATP酶）和Na（+）/HCO（3-）协同转运体1（NBC 1）的基因表达。在酸性淡水条件下，罗非鱼鳃NHE 3和NCC的mRNA表达量高于50 mM NaCl条件下，而V-ATPase和NBC 1的mRNA表达量差异不显著。此外，免疫细胞化学观察表明，在酸性淡水中，0 mM和50 mM NaCl的顶端-NHE 3 MR细胞扩大，并经常形成多细胞复合物，具有发达的深顶端开口。这些研究结果表明，鳃MR细胞响应外部盐度和pH值的处理，通过平行操纵的盐度调节和酸碱调节机制。(C)2010年爱思唯尔公司All rights reserved.
On exposure to hyposmotic acidic water, teleost fish suffer from decreases in blood osmolality and pH, and consequently activate osmoregulatory and acid-base regulatory mechanisms to restore disturbed ion and acid-base balances. In Mozambique tilapia Oreochromis mossambicus exposed to acidic (pH 4.0) or neutral (pH 7.4-7.7) freshwater in combination with 0mM or 50mM NaCl, we examined functional and morphological changes in gill mitochondria-rich (MR) cells. We assessed gene expression of Na(+)/H(+) exchanger-3 (NHE3), Na(+)/Cl(-) cotransporter (NCC), vacuolar-type H(+)-ATPase (V-ATPase) and Na(+)/HCO(3-) cotransporter-1 (NBC1) in the gills. The mRNA expression of NHE3 and NCC in tilapia gills were higher in acidic freshwater than in that supplemented with 50mM NaCl, while there was no significant difference in mRNA levels of V-ATPase and NBC1. In addition, immunocytochemical observations showed that apical-NHE3 MR cells were enlarged, and frequently formed multicellular complexes with developed deep apical openings in acidic freshwater with 0mM and 50mM NaCl. These findings suggest that gill MR cells respond to external salinity and pH treatments, by parallel manipulation of osmoregulatory and acid-base regulatory mechanisms. (C) 2010 Elsevier Inc. All rights reserved.