Evanescent-wave fluorescence microscopy using symmetric planar waveguides
Evanescent-wave fluorescence microscopy using symmetric planar waveguides
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DOI：
10.1364/oe.17.005075
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发表时间：
2009-03-30
期刊：
影响因子：
3.8
通讯作者：
Leosson, Kristjan
中科院分区：
文献类型：
--
作者：
Agnarsson, Bjoern;Ingthorsson, Saevar;Leosson, Kristjan
我们描述了一种新的倏逝波荧光激发方法，非常适合于生物样品的成像。激发光在平面光波导中传播，该平面光波导由夹在水溶液样品和具有匹配折射率的聚合物之间的薄波导芯组成，形成对称包层环境。与其他波导激励方法相比，这种结构具有明显的优势，如优越的图像质量、广泛的倏逝场穿透深度可调性以及与光纤的兼容性。该方法非常适合于培养细胞的细胞膜成像，包括细胞-细胞和细胞-基质相互作用，表面结合事件的监测以及涉及水溶液的类似应用。(C) 2009年美国光学学会
We describe a new evanescent-wave fluorescence excitation method, ideally suited for imaging of biological samples. The excitation light propagates in a planar optical waveguide, consisting of a thin waveguide core sandwiched between a sample in an aqueous solution and a polymer with a matching refractive index, forming a symmetric cladding environment. This configuration offers clear advantages over other waveguide-excitation methods, such as superior image quality, wide tunability of the evanescent field penetration depth and compatibility with optical fibers. The method is well suited for cell membrane imaging on cells in culture, including cell-cell and cell-matrix interaction, monitoring of surface binding events and similar applications involving aqueous solutions. (C) 2009 Optical Society of America