Teflon-coated silicon apertures for supported lipid bilayer membranes
Teflon-coated silicon apertures for supported lipid bilayer membranes
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用于支撑脂质双层膜的聚四氟乙烯涂层硅孔
DOI：
10.1063/1.1805712
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发表时间：
2004
影响因子：
4
通讯作者：
R. Eisenberg
中科院分区：
文献类型：
--
作者：
S. Wilk;M. Goryll;G. M. Laws;S. Goodnick;T. Thornton;M. Saraniti;J. Tang;R. Eisenberg
我们提出了一种使用众所周知的洁净室技术在硅基板上微加工孔径的方法，从而形成高度可重复的千兆密封电阻双层结构。使用等离子蚀刻机，在硅片上蚀刻出 150μm 的孔径。 Teflon™ 经过化学气相沉积，表面类似于块状 Teflon，并且具有疏水性。制造后，形成可重复的高电阻双层，并对自插入的单个 OmpF 孔蛋白离子通道蛋白进行特征测量。
We present a method for microfabricating apertures in a silicon substrate using well-known cleanroom technologies resulting in highly reproducible giga-seal resistance bilayer formations. Using a plasma etcher, 150μm apertures have been etched through a silicon wafer. Teflon™ has been chemically vapor deposited so that the surface resembles bulk Teflon and is hydrophobic. After fabrication, reproducible high resistance bilayers were formed and characteristic measurements of a self-inserted single OmpF porin ion channel protein were made.
影响因子：
3.4
作者：
Pantoja, R;Sigg, D;Heath, JR
通讯作者：
Heath, JR