INDUCTION OF BETA-GLUCOSIDASES IN NEUROSPORA-CRASSA
INDUCTION OF BETA-GLUCOSIDASES IN NEUROSPORA-CRASSA
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DOI：
10.1128/jb.116.1.295-303.1973
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发表时间：
1973-01-01
影响因子：
3.2
通讯作者：
BECK, RS
中科院分区：
文献类型：
--
作者：
EBERHART, BM;BECK, RS
研究了粗糙脉孢菌（Neurospora crassa）β-葡萄糖苷酶（EC 3.2.1.21）的诱导。纤维二糖对纤维二糖酶有诱导作用，其他诱导剂对该酶的诱导作用不大。在不存在二-β-葡萄糖苷诱导剂的情况下，纤维二糖酶活性在营养生命周期的所有阶段都非常低。在分生孢子形成之前的培养物生长后期，芳基-β-葡萄糖苷酶是不稳定的。在早期阶段，芳基-β-葡萄糖苷酶被纤维二糖、昆布二糖和龙胆二糖诱导，而被半乳糖、氨基糖和芳基-β-葡萄糖苷弱诱导。将β-葡萄糖苷酶的诱导特性与脉孢菌的其它双糖苷酶进行了比较。葡萄糖、2-脱氧-d-葡萄糖和乙酸钠可抑制β-葡萄糖苷酶的诱导。0.01和0.1 M之间的磷酸钠浓度刺激诱导的两种酶，而浓度高于0.1 M的抑制。两种β-葡萄糖苷酶的最适诱导条件均为pH6.0。在pH6.0 ~ 8.0范围内，纤维二糖酶的诱导作用受到的抑制程度大于芳基-β-葡萄糖苷酶。
The induction of β-glucosidases (EC 3.2.1.21) was studied inNeurospora crassa. Cellobiase was induced by cellobiose, but other inducers had little effect on this enzyme. Cellobiase activity was very low in all stages of the vegetative life cycle in the absence of di-β-glucoside inducer. Aryl-β-glucosidase was semiconstitutive at late stages of culture growth prior to conidiation. At early stages, aryl-β-glucosidase was induced by cellobiose, laminaribiose, and gentiobiose, and weakly induced by galactose, amino sugars, and aryl-β-glucosides. The induction properties of the β-glucosidases are compared with those of the other disaccharidases ofNeurospora. The induction of β-glucosidases was inhibited by glucose, 2-deoxy-d-glucose, and sodium acetate. Sodium phosphate concentrations between 0.01 and 0.1 M stimulated induction of both enzymes, while concentrations above 0.1 M were inhibitory. The optimal condition for induction of both β-glucosidases was pH 6.0. Cellobiase induction was relatively more inhibited than aryl-β-glucosidase in the range of pH 6.0 to 8.0.