Variable number of tandem aminoacid repeats in adhesion-related CDS products in Mycoplasma hyopneumoniae strains
Variable number of tandem aminoacid repeats in adhesion-related CDS products in Mycoplasma hyopneumoniae strains
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DOI：
10.1016/j.vetmic.2006.04.022
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发表时间：
2006-09-10
影响因子：
3.3
通讯作者：
Ferreira, Henrique Bunselmeyer
中科院分区：
文献类型：
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作者：
de Castro, Luiza Amaral;Pedroso, Thiago Rodrigues;Ferreira, Henrique Bunselmeyer
猪肺炎支原体基因组包含至少22个区域，在编码DNA序列（CDS）内具有可变数量的串联核苷酸重复序列（VNTR）。在这项工作中，VNTR-含CDS进行了分析，以评估其变异程度，可能与抗原特性的相关性，以及它们的潜力，作为一个菌株分型PCR检测的基础。我们已经分析了五个M中的VNTR。猪肺炎链球菌菌株（J，7448，7422，PMS，和232），基于公开的基因组序列和扩增和测序的DNA片段。这些VNTR分布在12个基因中，其中大多数编码推定的表面蛋白，包括已知的粘附素。在任何VNTR中的重复单元的数目在所分析的菌株之间是高度可变的，但是它们无一例外地在读码框内，并且因此编码可变数目的氨基酸重复（VNTAR）。这些VNTAR决定了相应蛋白质中推定的结构、物理化学和抗原变异，对与M.在某些实施方案中，所述方法包括抑制细菌的致病性，例如细胞粘附和与宿主免疫系统的相互作用。考虑到所表征的VNTAR至少在体外是相对稳定的，并且它们的大小是菌株特异性的，我们开发了用于M.猪肺炎病毒株鉴定，可用于地方性肺炎（EP）诊断、菌株分型和区分接种EP疫苗的动物中的流行田间分离株与疫苗菌株。(c)2006 Elsevier B. V.保留所有权利。
The Mycoplasma hyopneumoniae genome contains at least 22 regions with a variable number of tandem nucleotide repeats (VNTRs) within coding DNA sequences (CDSs). In this work, the VNTR-containing CDSs were analysed in order to evaluate their degree of variation, possible correlations with antigenic properties, and their potential to be used as a basis for a strain typing PCR assay. We have analysed the VNTRs in five M. hyopneumoniae strains (J, 7448, 7422, PMS, and 232), based on published genomic sequences and on amplified and sequenced DNA segments. These VNTRs are distributed among 12 genes, most of which encode putative surface proteins, including known adhesins. The number of repeat units in any of the VNTRs is highly variable among the analysed strains, but they are, without exception, translationally in frame, and, therefore, code for a variable number of aminoacid repeats (VNTARs). These VNTARs determine putative structural, physicochemical and antigenic variations in the corresponding proteins, with potential implications for aspects associated to M. hyopneumoniae pathogenicity, such as cell adhesion and interactions with the host immune system. Considering that the characterized VNTARs are relatively stable, at least in vitro, and their sizes are strain-specific, we have developed a VNTR-based PCR assay for M. hyopneumoniae strain identification, useful for enzootic pneumonia (EP) diagnosis, strain typing, and distinction of circulating field isolates from vaccine strains in animals vaccinated against EP. (c) 2006 Elsevier B.V. All rights reserved.