Larval Testes of the Tobacco Budworm: A New Source of Insect Ecdysteroids
Larval Testes of the Tobacco Budworm: A New Source of Insect Ecdysteroids
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烟草芽虫幼虫睾丸：昆虫蜕皮类固醇的新来源
DOI：
10.1126/science.218.4575.896
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发表时间：
1982
期刊：
影响因子：
56.9
通讯作者：
Alexej B. Boakovec
中科院分区：
文献类型：
--
作者：
M. Loeb;C. W. Woods;Ethel P. Brandt;Alexej B. Boakovec
烟青虫末龄幼虫的睾丸在 2.5 小时内向培养介质中释放的蜕皮类固醇（通过放射免疫测定法判断）是睾丸匀浆中的五倍。睾丸成分的孵化表明睾丸鞘可能是蜕皮类固醇合成的部位。通过高效液相色谱法对血淋巴、睾丸匀浆和培养介质进行分级分离，在每种情况下都会产生独特的蜕皮类固醇模式。因此，释放的睾丸蜕皮激素可能转化为其他形式以供使用、隔离或全身循环。它们的功能未知。
Testes of last-instar larvae of the tobacco budworm release five times more ecdysteroid into incubation medium (judged by radioimmunoassay) in 2.5 hours than is found in testis homogenates. Incubation of testicular components indicates that the testis sheath may be the site of ecdysteroid synthesis. Fractionation of hemalymph, testis homogenate, and incubation medium by high-performance liquid chromatography produces a distinct ecdysteroid pattern in each case. Thus, released testis ecdysteroids are probably converted to other forms for use, sequestration, or general circulation. Their functions are unknown.