Effects of supplementation with free radical scavengers on the survival and fertilization rates of mouse cryopreserved oocytes
Effects of supplementation with free radical scavengers on the survival and fertilization rates of mouse cryopreserved oocytes
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DOI：
10.1093/humrep/16.9.1976
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发表时间：
2001-09-01
影响因子：
6.1
通讯作者：
Ishikawa, M
中科院分区：
文献类型：
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作者：
Dinara, S;Sengoku, K;Ishikawa, M
背景技术背景：本研究旨在探讨添加自由基清除剂对小鼠卵母细胞冻融后存活率和受精率的影响。方法：将卵丘细胞与超数排卵卵母细胞在丙二醇中缓慢冷冻结合快速解冻方案进行冷冻保存。冷冻保存培养基中补充了抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶，并与一氧化氮（NO）清除剂，血红蛋白（Hb）。结果：添加50 IU/ml SOD组小鼠的存活率和受精率均显著高于对照组（P
BACKGROUND: This study was conducted to investigate the effects of supplementation with free radical scavengers on the survival and fertilization rates of freeze-thawed mouse oocytes. METHODS: Superovulated oocytes with cumulus cells were cryopreserved by slow freezing in propanediol combined with a rapid thawing protocol. The cryopreservation medium was supplemented with the antioxidant enzymes superoxide dismutase (SOD) and catalase, and with the nitric oxide (NO) scavenger, haemoglobin (Hb). RESULTS: The addition of 50 IU/ml SOD showed significantly higher survival and fertilization capabilities compared with control (P