A sensitive electrochemical method based on Fenton-induced DNA oxidation for detection of hydroxyl radical
A sensitive electrochemical method based on Fenton-induced DNA oxidation for detection of hydroxyl radical
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DOI：
10.1039/c4ay00843j
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发表时间：
2014-07
影响因子：
3.1
通讯作者：
J. Si;Lu Lu-Lu;Z. Gao;Yu Zhang;H. Luo;N. Li
中科院分区：
文献类型：
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作者：
J. Si;Lu Lu-Lu;Z. Gao;Yu Zhang;H. Luo;N. Li
利用芬顿反应产生的羟基自由基（stecOH），研制了一种灵敏、选择性好的生物传感器。stecOH可氧化损伤DNA并将其切割成碎片。在此基础上，以[Ru（NH3）6]3+为电化学指示剂，在自组装DNA的金电极上测定了stecOH。所得的电化学生物传感器显示出从125 pM到625 nM的宽的检测线性范围，检测限为80 pM和令人满意的选择性。
A sensitive and selective biosensor has been developed to detect the hydroxyl radical (˙OH), which was generated through the Fenton reaction. ˙OH could oxidatively damage DNA and cleave it into pieces. Based on this, taking [Ru(NH3)6]3+ as the electrochemical indicator, ˙OH was determined on a gold electrode with self-assembled DNA. The resulting electrochemical biosensor showed a wide linear range of detection from 125 pM to 625 nM with a detection limit of 80 pM and satisfactory selectivity.