Direct evidence of multicompartment aggregates in polyelectrolyte-charged liposome complexes
Direct evidence of multicompartment aggregates in polyelectrolyte-charged liposome complexes
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DOI：
10.1529/biophysj.106.085142
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发表时间：
2006-08-15
影响因子：
3.4
通讯作者：
Diociaiuti, M.
中科院分区：
文献类型：
--
作者：
Bordi, F.;Cametti, C.;Diociaiuti, M.
通过动态光散射和透射电子显微镜测量相结合的方法，我们提供了在多离子诱导的脂质体聚集中存在平衡团簇相的直接证据，其中脂质体保持其完整性，并能够保持外部介质中的水核含量。我们制备了内部含有不同浓度(分别为0.1M和0.01M)的不同CsC l电解质溶液的单一脂质体。在多离子诱导的这两种不同载药量的脂质体混合物的络合过程中，形成可逆的聚集体，其多室结构揭示了非融合脂质体的同时存在。由介观大小的囊泡组成的团簇，由带电的脂类与相反电荷的多离子耦合而实现，作为软物质中的各种现象的模型系统，以及它们作为药物输送系统在生物医学应用中的潜在应用，正发挥着越来越重要的作用。如本文所述的脂质体聚集体，其中静电相互作用是促进聚集的主要驱动力，可能代表了一类新的有趣的胶体，它产生了丰富的现象学，具有几种值得进一步研究的不同寻常的胶体行为。
By means of the combined use of dynamic light scattering and transmission electron microscopy measurements, we provide a direct evidence for the existence of an equilibrium cluster phase in the polyion-induced liposome aggregation, where the liposomes maintain their integrity, with the ability of preserving the aqueous core content from the external medium. We prepared single liposomes containing, in their interior, different CsCl electrolyte solutions at different concentrations (0.1 and 0.01 M, respectively). During the polyion-induced complexation of a mixture of these two differently loaded liposomes, reversible aggregates form, whose multicompartmental structure reveals the simultaneous presence of nonfused liposomes. Clusters composed by mesoscopic-sized vesicles and realized by charged lipids coupled to oppositely charged polyions are playing an increasingly important role as model systems in a variety of phenomena in soft matter and for their potential use in biomedical applications as drug delivery systems. Aggregates of liposomes such as those described in this article, where the electrostatic interactions are the primary driving forces promoting aggregation, may represent a new and interesting class of colloids which give rise to a rich phenomenology with several unusual colloidal behaviors that deserve to be further investigated.