Mouse cyp2g1 gene: promoter structure and tissue-specific expression of a cyp2g1-lacz fusion gene in transgenic mice.
Mouse cyp2g1 gene: promoter structure and tissue-specific expression of a cyp2g1-lacz fusion gene in transgenic mice.
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小鼠 cyp2g1 基因：转基因小鼠中 cyp2g1-lacz 融合基因的启动子结构和组织特异性表达。
DOI：
10.1006/abbi.2001.2410
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Ding,X
中科院分区：
文献类型：
--
作者：
Zhuo,X;Schwob,JE;Swiatek,PJ;Ding,X
确定小鼠Cyp 2g 1基因的结构，以确定其嗅觉粘膜特异性表达的重要调控区域。分离并鉴定了两个Cyp 2g 1基因组克隆。利用3.6-腺苷酸酶5′侧翼序列制备Cyp 2g 1-LacZ融合基因，用于转基因小鼠的生产。通过组织提取物中的β-半乳糖苷酶活性测定，在5个不同的转基因品系中，在嗅粘膜中检测到转基因表达，但在检查的任何其他组织中均未检测到。因此，3.6-乙酰氨基转移酶片段含有足够的调节元件，嗅觉粘膜特异性和适当的发育报告基因的表达。然而，组织学和免疫组织化学研究表明，在嗅粘膜中的转基因的表达是斑片状的和细胞的表达模式的转基因并不完全匹配的内源性基因。这些结果暗示存在额外的调控序列，是必要的正确的细胞类型选择性内的嗅粘膜。
The structure of the mouse Cyp2g1 gene was determined to identify regulatory regions important for its olfactory mucosa-specific expression. Two Cyp2g1 genomic clones were isolated and characterized. A 3.6-kilobase 5′-flanking sequence was used to prepare a Cyp2g1–LacZ fusion gene for transgenic mice production. Transgene expression, as determined by β-galactosidase activity in tissue extracts, was detected in the olfactory mucosa, but not in any other tissues examined, in five different transgenic lines. Thus, the 3.6-kilobase fragment contained regulatory elements sufficient for olfactory mucosa-specific and proper developmental expression of the reporter gene. However, histological and immunohistochemical studies indicated that the expression of the transgene in the olfactory mucosa was patchy and the cellular expression patterns of the transgene did not exactly match that of the endogenous gene. These results implicate the presence of additional regulatory sequences that are necessary for the correct cell type-selectivity within the olfactory mucosa.