Zerumbone inhibits epithelial-mesenchymal transition and cancer stem cells properties by inhibiting the β-catenin pathway through miR-200c (Retracted article. See vol. 237, pg. 2602, 2022)
Zerumbone inhibits epithelial-mesenchymal transition and cancer stem cells properties by inhibiting the β-catenin pathway through miR-200c (Retracted article. See vol. 237, pg. 2602, 2022)
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DOI：
10.1002/jcp.26874
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发表时间：
2018-12-01
影响因子：
5.6
通讯作者：
Najafi, Rezvan
中科院分区：
文献类型：
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作者：
Dermani, Fatemeh Karimi;Amini, Razieh;Najafi, Rezvan
结直肠癌（CRC）是世界上最致命和最猖獗的人类恶性肿瘤之一。Zerumbone是一种从亚热带生姜中分离出来的倍半萜，已被发现对各种癌症都有抗肿瘤作用。然而，Zerumbone对结直肠癌生物学特性的影响，包括上皮-间充质转化（EMT）和癌症干细胞（CSCs）的影响尚未完全阐明。在这里，我们研究了在miR-200c存在或不存在的情况下，Zerumbone对EMT过程、CSC标记物和-catenin信号通路的抑制作用。采用3-（4,5 -二甲基噻唑-2-基）- 2,5 -二苯基溴化四氮唑法检测Zerumbone对HCT-116和SW-48细胞活力的影响。探讨Zerumbone对emt相关基因、CSCs标志物、细胞迁移、侵袭、成球和连环蛋白信号通路的影响。为了评估miR-200c在Zerumbone抗癌作用中的作用，我们通过na -anti-miR-200c下调miR-200c。Zerumbone显著抑制HCT-116和SW-48细胞系的细胞活力、迁移、侵袭和成球潜能。Zerumbone显著抑制了EMT和CSC特性，并下调了-catenin。miR200c的沉默降低了Zerumbone对CRC细胞中EMT和CSCs的抑制作用。这些数据表明，Zerumbone可能是通过miR-200c抑制-catenin通路来降低CRC进展风险的有希望的候选药物。
Colorectal cancer (CRC) is one of the most lethal and rampant human malignancies in the world. Zerumbone, a sesquiterpene isolated from subtropical ginger, has been found to exhibit an antitumor effect in various cancer types. However, the effect of Zerumbone on the biological properties of CRC, including epithelial-mesenchymal transition (EMT) and cancer stem cells (CSCs) has not been fully elucidated. Here, we investigated the inhibitory action of Zerumbone on the EMT process, CSC markers, and the -catenin signaling pathway in the presence or absence of miR-200c. The effect of Zerumbone on HCT-116 and SW-48 cells viability was examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effects of Zerumbone on EMT-related genes, CSCs markers, cell migration, invasion, sphere-forming, and-catenin signaling pathway were explored. To evaluate the role of miR-200c in anticancer effects by Zerumbone, miR-200c was downregulated by LNA-anti-miR-200c. Zerumbone significantly inhibited cell viability, migration, invasion, and sphere-forming potential in HCT-116 and SW-48 cell lines. Zerumbone significantly suppressed the EMT and CSC properties as well as downregulated the -catenin. Silencing of miR200c reduced the inhibitory effects of Zerumbone on EMT and CSCs in CRC cells. These data indicated that Zerumbone may be a promising candidate for reducing the risk of CRC progression by suppressing the -catenin pathway via miR-200c.