A phosphatidylinositol transfer protein controls the phosphatidylcholine content of yeast Golgi membranes.
A phosphatidylinositol transfer protein controls the phosphatidylcholine content of yeast Golgi membranes.
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DOI：
10.1083/jcb.124.3.273
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发表时间：
1994-02
影响因子：
7.8
通讯作者：
Bankaitis, V A
中科院分区：
文献类型：
--
作者：
McGee, T P;Skinner, H B;Whitters, E A;Henry, S A;Bankaitis, V A
SEC14p是酵母高尔基复合体中蛋白质运输所必需的。我们描述了在高尔基分泌功能与其通常的SEC14p要求解耦的条件下酵母体膜和高尔基膜磷脂组成的定量分析。这些数据表明，SEC14p特异性地维持高尔基膜中磷脂酰胆碱含量的降低，并且表明SEC14p的过量产生显着降低了体内通过cdp -胆碱途径合成磷脂酰胆碱的表观速率。我们认为SEC14p作为高尔基膜磷脂组成的传感器，通过其调节高尔基膜中cdp -胆碱途径的活性，从而维持与这些膜的基本分泌功能相容的磷脂酰胆碱含量。
SEC14p is required for protein transport from the yeast Golgi complex. We describe a quantitative analysis of yeast bulk membrane and Golgi membrane phospholipid composition under conditions where Golgi secretory function has been uncoupled from its usual SEC14p requirement. The data demonstrate that SEC14p specifically functions to maintain a reduced phosphatidylcholine content in Golgi membranes and indicate that overproduction of SEC14p markedly reduces the apparent rate of phosphatidylcholine biosynthesis via the CDP-choline pathway in vivo. We suggest that SEC14p serves as a sensor of Golgi membrane phospholipid composition through which the activity of the CDP-choline pathway in Golgi membranes is regulated such that a phosphatidylcholine content that is compatible with the essential secretory function of these membranes is maintained.