A Two-stage pH and Temperature Control with Substrate Feeding Strategy for Production of Gamma-aminobutyric Acid by Lactobacillus brevis CGMCC 1306
A Two-stage pH and Temperature Control with Substrate Feeding Strategy for Production of Gamma-aminobutyric Acid by Lactobacillus brevis CGMCC 1306
复制标题
采用底物补料策略的两阶段 pH 和温度控制，用于短乳杆菌 CGMCC 1306 生产 γ-氨基丁酸
DOI：
10.1016/s1004-9541(13)60568-6
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发表时间：
2013-10-01
影响因子：
3.8
通讯作者：
Mei Lehe
中科院分区：
文献类型：
--
作者：
Peng Chunlong;Huang Jun;Mei Lehe
研究了短乳杆菌（Lactobacillus brevis）生产γ-氨基丁酸（GABA）的优化方法。结果表明，pH5.0时细胞生长最快，而pH4.5时细胞生长最快。细胞生长的最适温度（35 ℃）低于GABA形成的最适温度（40 ℃）。在两阶段pH和温度控制发酵中，培养物在pH5.0和35 ℃下保持32 h，然后调节到pH4.5和40 ℃，GABA产量显著增加，达到474.79mmol。L ~（-1），72 h时为398.63 mmol . L ~（-1），采用一级pH和温度控制工艺，将培养条件恒定控制在pH5.0和35 ℃。为了避免高浓度谷氨酸（L-MSG）对细胞生长的抑制作用，采用两段补料控制发酵的方法，分别在接种后32 h和56 h向摇瓶中补加106.87 mmol（20 g）L-MSG。GABA浓度达到526.33 mmol . L-1，发酵量提高了38%。这些结果为实现L. brevis CGMCC 1306.
Methods to optimize the production of gamma-aminobutyric acid (GABA) by Lactobacillus brevis CGMCC 1306 were investigated. Results indicated that cell growth was maximal at pH 5.0, while pH 4.5 was preferable to GABA formation. The optimal temperature for cell growth (35 degrees C) was lower than that for GABA formation (40 degrees C). In a two-stage pH and temperature control fermentation, cultures were maintained at pH 5.0 and 35 degrees C for 32 h, then adjusted to pH 4.5 and 40 C, GABA production increased remarkably and reached 474.79 mmol . L-1 at 72 h, while it was 398.63 mmol . L-1 with one stage pH and temperature control process, in which cultivation conditions were constantly controlled at pH 5.0 and 35 C. In order to avoid the inhibition of cell growth at higher L-monosodium glutamate (L-MSG) concentrations, the two-stage control fermentation with substrate feeding strategy was applied to GABA production, with 106.87 mmol (20 g) L-MSG supplemented into the shaking-flask at 32 h and 56 h post-inoculation separately. The GABA concentration reached 526.33 mmol . L-1 at 72 h with the fermentation volume increased by 38%. These results will provide primary data to realize large-scale production of GABA by L. brevis CGMCC 1306.