Proteomic approaches in circadian biology.
Proteomic approaches in circadian biology.
复制标题
DOI：
10.1007/978-3-642-25950-0_17
复制
发表时间：
2013-01-01
影响因子：
--
通讯作者：
Mann, Matthias
中科院分区：
文献类型：
--
作者：
Robles, Maria S;Mann, Matthias
生物钟是一种内源性振荡器，它驱动着一系列基因的节律表达，这些基因协调着新陈代谢和生理学。最近的证据表明，转录后和翻译后机制在调节昼夜节律基因表达，特别是生物钟的分子机制中起着至关重要的作用。与长期以来用于研究昼夜节律生物学的遗传技术相比，蛋白质组学方法迄今为止受到限制，并且如果应用的话，则使用二维凝胶电泳（2-DE）。在这里，我们回顾了蛋白质组学方法应用到日期在昼夜节律领域，我们还讨论了使用尖端的蛋白质组学技术在昼夜节律生物学令人兴奋的潜力。大规模、定量的蛋白质丰度测量将有助于理解生物钟在多大程度上驱动转录调控下游的蛋白质丰度的系统性节律。
Circadian clocks are endogenous oscillators that drive the rhythmic expression of a broad array of genes that orchestrate metabolism and physiology. Recent evidence indicates that posttranscriptional and posttranslational mechanisms play essential roles in modulating circadian gene expression, particularly for the molecular mechanism of the clock. In contrast to genetic technologies that have long been used to study circadian biology, proteomic approaches have so far been limited and, if applied at all, have used two-dimensional gel electrophoresis (2-DE). Here, we review the proteomics approaches applied to date in the circadian field, and we also discuss the exciting potential of using cutting-edge proteomics technology in circadian biology. Large-scale, quantitative protein abundance measurements will help to understand to what extent the circadian clock drives system wide rhythms of protein abundance downstream of transcription regulation.