Assays for Apical Constriction Using the Xenopus Model.
Assays for Apical Constriction Using the Xenopus Model.
复制标题
DOI：
10.1007/978-1-0716-2035-9_24
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
顶端缩窄是指主动的肌动球蛋白驱动的过程，其减少上皮细胞中的顶端细胞表面积。顶端缩窄在胚胎发育过程中的上皮形态发生中有多种用途，如原肠胚形成、神经管闭合和器官发生。心尖缩窄的缺陷可导致先天性出生缺陷，但我们对心尖缩窄的分子控制的了解相对有限。为了发现新的顶端收缩的遗传调节因子，并获得对这一过程的细胞生物学的机械见解，我们需要可靠的检测系统，允许实时观察和定量的顶端收缩，因为它发生，并允许增益和损失的功能分析，以探索基因功能和顶端收缩过程中的相互作用。在这一章中，我们描述了使用早期非洲爪蟾胚胎作为一个检测系统，以调查在原肠胚和神经胚形成过程中参与顶端收缩的分子机制。
Apical constriction refers to the active, actomyosin-driven process that reduces apical cell surface area in epithelial cells. Apical constriction is utilized in epithelial morphogenesis during embryonic development in multiple contexts, such as gastrulation, neural tube closure, and organogenesis. Defects in apical constriction can result in congenital birth defects, yet our understanding of the molecular control of apical constriction is relatively limited. To uncover new genetic regulators of apical constriction and gain mechanistic insight into the cell biology of this process, we need reliable assay systems that allow real-time observation and quantification of apical constriction as it occurs and permit gain- and loss-of-function analyses to explore gene function and interaction during apical constriction. In this chapter, we describe using the early Xenopus embryo as an assay system to investigate molecular mechanisms involved in apical constriction during both gastrulation and neurulation.