Critical Role of ESCRT Machinery in EGFR Recycling
Critical Role of ESCRT Machinery in EGFR Recycling
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DOI：
10.1021/bi900865u
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发表时间：
2009-10-13
期刊：
影响因子：
2.9
通讯作者：
Raymond, John R., Sr.
中科院分区：
文献类型：
--
作者：
Baldys, Aleksander;Raymond, John R., Sr.
近年来，EGFR囊泡转运至溶酶体的分子机制受到了相当大的关注。现在很清楚，运输所需的内体分选复合物（escrt）对EGFR降解至关重要。尽管越来越多的膜受体也通过特定途径进行再循环，但关于EGFR的调节再循环的信息很少。在这项研究中，我们研究了escrt在双调节蛋白（AR）刺激后EGFR循环中的作用。我们使用ESCRT小干扰RNA （siRNA）双链来证明ar诱导的EGFR细胞内加工涉及通过ESCRT家族的特定成员主动分选到回收途径。
The molecular mechanisms of EGFR vesicular trafficking to lysosomes have recently received considerable attention. It is now clear that endosomal sorting complexes required for transport (ESCRTs) are critical for EGFR degradation. Although an increasing number of membrane receptors also undergo recycling via specific pathways, little information is available regarding regulated recycling of EGFR. In this study, we investigated the roles of ESCRTs in EGFR recycling after stimulation with amphiregulin (AR). We used ESCRT small interfering RNA (siRNA) duplexes to demonstrate that AR-induced EGFR intracellular processing involves active sorting to the recycling pathway through specific members of the ESCRT family.