PM2.5 and the typical components cause organelle damage, apoptosis and necrosis: Role of reactive oxygen species.
PM2.5 and the typical components cause organelle damage, apoptosis and necrosis: Role of reactive oxygen species.
复制标题
PM2.5及其典型成分引起细胞器损伤、细胞凋亡和坏死：活性氧的作用。
DOI：
10.1016/j.scitotenv.2021.146785
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发表时间：
2021-03
影响因子：
9.8
通讯作者：
Wei Jiang
中科院分区：
文献类型：
--
作者：
Xifeng Shan;Ling Liu;Gang Li;Kexin Xu;Bingyan Liu;Wei Jiang
In this research, the organelle damage, apoptosis and necrosis induced by PM2.5, BC and Kaolin were studied using human bronchial epithelial (16HBE) cells. PM2.5, BC and Kaolin all induce cell death, LDH release and excess intracellular ROS generation. For the organelle injuries, Kaolin and high-dose PM2.5(240 μg/mL) cause lysosomal acidification, but BC causes lysosomal alkalization (lysosomal membrane permeabilization, LMP). BC and Kaolin cause the loss of mitochondrial membrane potential (MMP), while PM2.5does not. For the cell death mode, PM2.5causes both apoptosis and necrosis. However only necrosis has been detected in the BC and Kaolin treated groups, indicating the more severe cellular insult. Excess ROS generation is involved in the organelle damage and cell death. ROS contributes to the BC-induced LMP and necrosis, but does not significantly affect the Kaolin-induced MMP loss and necrosis. Therefore, the BC component in PM2.5may cause cytotoxicity via ROS-dependent pathways, the Kaolin component may damage cells via ROS-independent mechanisms such as strong interaction. The PM2.5-induced apoptosis and necrosis can be partially mitigated after the removal of ROS, indicating the existence of both the ROS-dependent and ROS-independent mechanisms due to the complicated PM2.5components. BC represents the anthropogenic source component in PM2.5, while Kaolin represents the natural source component. Our results provide knowledge on the toxic mechanisms of typical PM2.5components at the cellular and subcellular levels.
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影响因子：
10
作者：
Vuong NQ;Breznan D;Goegan P;O'Brien JS;Williams A;Karthikeyan S;Kumarathasan P;Vincent R
通讯作者：
Vincent R
影响因子：
2.9
作者：
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通讯作者：
Min Wei;Zhenfa Fu;Che Wang;Wei Zheng;Song Li;W. Le
影响因子：
8.8
作者：
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V. Verma;S. Subbiah;S. Kota
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影响因子：
8.8
作者：
Chen, Qi;Luo, Xiao-San;Jin, Ling
通讯作者：
Jin, Ling
影响因子：
2.9
作者：
Anil, Ismail;Golcuk, Kurtulus;Karaca, Ferhat
通讯作者：
Karaca, Ferhat