Spontaneous neoplastic evolution of Chinese hamster cells in culture: multistep progression of phenotype.
Spontaneous neoplastic evolution of Chinese hamster cells in culture: multistep progression of phenotype.
复制标题
中国仓鼠细胞培养的自发肿瘤进化：表型的多步进展。
作者：
P. Kraemer;G. Travis;F. Ray;Scott Cram
中国仓鼠细胞株，每个来自不同的胎儿，在培养中被携带，并在不同传代水平上测试肿瘤的致瘤性和体外肿瘤指标。在没有任何治疗的情况下，所有20个这样的谱系都产生了永久细胞系，并显示出肿瘤进展的其他迹象。采用明胶海绵预植入法监测裸鼠体内的致瘤性。体外自发肿瘤进展过程可分为四个阶段。根据传代水平衡量，这些阶段的进展速度在不同的独立谱系之间差异极大。对一个谱系的详细研究表明，通常情况下，转化指标与致瘤性相关，但并不表明癌前永久谱系是否会迅速进展。
Chinese hamster cell strains, each initiated from a separate fetus, were carried in culture and tested for tumorigenicity and in vitro indicators of neoplasia at various passage levels. In the absence of any treatment, all 20 such lineages yielded permanent cell lines and showed other indications of neoplastic progression. A preimplanted gelatin sponge assay method was used to monitor tumorigenicity in nude mice. The process of spontaneous neoplastic progression in vitro could be divided into four stages. The rate of progression through these stages, as measured by passage level, was extremely variable between independent lineages. Detailed studies of one lineage showed that transformation indicators, in general, correlated with tumorigenicity but did not indicate whether or not a preneoplastic, permanent lineage would rapidly progress.
中国仓鼠细胞培养的自发肿瘤进化：核型的多步进展。
影响因子：
11.2
作者：
Cram,LS;Bartholdi,MF;Ray,FA;Travis,GL;Kraemer,PM
通讯作者：
Kraemer,PM