The tomato E8 gene influences ethylene biosynthesis in fruit but not in flowers
The tomato E8 gene influences ethylene biosynthesis in fruit but not in flowers
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DOI：
10.1104/pp.112.2.537
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发表时间：
1996-10-01
期刊：
影响因子：
7.4
通讯作者：
Deikman, J
中科院分区：
文献类型：
--
作者：
Kneissl, ML;Deikman, J
我们研究了番茄（Lycopersicon esculentum）E8基因的功能。以前的实验中，使用的反义抑制E8表明，E8蛋白在果实中乙烯的进化有负面影响。E8在花和果实中表达，并且其在花药中的表达较高。我们将花椰菜花叶病毒35 S-E8基因导入番茄植株中，获得了E8过表达的植株和E8表达由于共抑制而被抑制的植株。E8在未成熟果实中的过表达并不影响果实成熟过程中乙烯释放的水平，然而，共抑制E8蛋白的减少确实导致成熟过程中乙烯释放水平升高。乙烯，1-氨基环丙烷-1-羧酸（ACC），和ACC氧化酶的mRNA水平增加约7倍，在果实中的植物减少E8蛋白。ACC合成酶2 mRNA水平增加2.5倍，ACC合成酶4 mRNA不受影响。花药中E8蛋白的减少并不影响ACC或编码乙烯生物合成酶的mRNA的积累。我们的研究结果表明，在果实成熟过程中，E8反应的产物参与乙烯生物合成的反馈调节。
We investigated the function of the tomato (Lycopersicon esculentum) E8 gene. Previous experiments in which antisense suppression of E8 was used suggested that the E8 protein has a negative effect on ethylene evolution in fruit. E8 is expressed in flowers as well as in fruit, and its expression is high in anthers. We introduced a cauliflower mosaic virus 35S-E8 gene into tomato plants and obtained plants with overexpression of E8 and plants in which E8 expression was suppressed due to co-suppression. Overexpression of E8 in unripe fruit did not affect the level of ethylene evolution during fruit ripening; however, reduction of E8 protein by cosuppression did lead to elevated levels during ripening. Levels for ethylene, 1-aminocyclopropane-1-carboxylic acid (ACC), and ACC oxidase mRNA were increased approximately 7-fold in fruit of plants with reduced E8 protein. Levels of ACC synthase 2 mRNA were increased 2.5-fold, and ACC synthase 4 mRNA was not affected. Reduction of E8 protein in anthers did not affect the accumulation of ACC or of mRNAs encoding enzymes involved in ethylene biosynthesis. Our results suggest that the product of the E8 reaction participates in feedback regulation of ethylene biosynthesis during fruit ripening.