Ultrasensitive Assay for Telomerase Activity via Self-Enhanced Electrochemiluminescent Ruthenium Complex Doped Metal-Organic Frameworks with High Emission Efficiency.
Ultrasensitive Assay for Telomerase Activity via Self-Enhanced Electrochemiluminescent Ruthenium Complex Doped Metal-Organic Frameworks with High Emission Efficiency.
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DOI：
10.1021/acs.analchem.7b00259
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发表时间：
2017-02
影响因子：
7.4
通讯作者：
C. Xiong;W. Liang;Ying-Ning Zheng;Ying Zhuo;Y. Chai;R. Yuan
中科院分区：
文献类型：
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作者：
C. Xiong;W. Liang;Ying-Ning Zheng;Ying Zhuo;Y. Chai;R. Yuan
本文以自增强型钌聚乙烯亚胺（Ru-PEI）配合物掺杂的咪唑骨架分子8（Ru-PEI@ZIF-8）为电化学发光指示剂，采用酶辅助DNA循环扩增策略，构建了一种高灵敏度的“关-开”电化学发光（ECL）生物传感器。通过Ru-PEI络合物自增强掺杂，在ZIF-8分子筛的生长过程中，合成了Ru-PEI@ZIF-8纳米复合材料，该复合材料具有高的电致发光效率和优异的稳定性。此外，由于Ru-PEI@ZIF-8的多孔性，Ru-PEI@ZIF-8的外层和内层中的自增强Ru-PEI复合物可以被电子激发，使得自增强ECL材料的利用率显著提高。为了进一步提高所提出的生物传感器的灵敏度，端粒酶活性信号转化为触发DNA信号，其进一步通过酶辅助的DNA双链扩增策略进行扩增。该电化学发光生物传感器对5 × 101 ~ 106个Hela细胞的端粒酶活性检测具有良好的性能，检测限为11个细胞。将该方法应用于肿瘤细胞端粒酶活性的检测，结果表明该方法具有潜在的应用价值，可作为抗癌药物筛选的评价工具。
Here, an ultrasensitive "off-on" electrochemiluminescence (ECL) biosensor was proposed for the determination of telomerase activity by using a self-enhanced ruthenium polyethylenimine (Ru-PEI) complex doped zeolitic imidazolate framework-8 (Ru-PEI@ZIF-8) with high ECL efficiency as an ECL indicator and an enzyme-assisted DNA cycle amplification strategy. The Ru-PEI@ZIF-8 nanocomposites were synthesized by self-enhanced Ru-PEI complex doping during the growth of zeolitic imidazolate framework-8 (ZIF-8), which presented high ECL efficiency and excellent stability. Furthermore, owing to the porosity of Ru-PEI@ZIF-8, the self-enhanced Ru-PEI complex in the outer layer and inner layer of self-enhanced Ru-PEI@ZIF-8 could be excited by electrons causing the utilization ratio of the self-enhanced ECL materials to be remarkably increased. To further improve the sensitivity of the proposed biosensor, the telomerase activity signal was converted into the trigger DNA signal which was further amplified by an enzyme-assisted DNA recycle-amplification strategy. The proposed ECL biosensor presented great performance for telomerase activity detection from 5 × 101 to 106 Hela cells with a detection limit of 11 cells. Moreover, this method was applied in the detection of telomerase activity from cancer cells treated with an anticancer drug, which indicated the proposed method held potential application value as an evaluation tool in anticancer drug screening.