DISTINGUISHING BETWEEN NITRIFICATION AND DENITRIFICATION AS SOURCES OF GASEOUS NITROGEN-PRODUCTION IN SOIL

DISTINGUISHING BETWEEN NITRIFICATION AND DENITRIFICATION AS SOURCES OF GASEOUS NITROGEN-PRODUCTION IN SOIL
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DOI:
10.1128/aem.52.6.1280-1286.1986
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发表时间:
1986-12-01
影响因子:
4.4
通讯作者:
PERRY, TO
PERRY, TO
中科院分区:
生物学2区
文献类型:
--
作者:
DAVIDSON, EA;SWANK, WT;PERRY, TO

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土壤中产生的N2O的来源往往是不确定的,因为反硝化和硝化可以同时发生在同一个土壤团聚体。提出了一种技术,利用这些过程的差异敏感性C2H2抑制区分气态氮损失从土壤中。反硝化N2O估计从24小时的实验室培养,其中硝化被抑制10帕C2H2。硝化N2O的估计从N2O生产下没有C2H2和反硝化测定之间的差异。反硝化N_2是根据10-kPa C_2H_2和10 Pa下N_2O产生量的差异估算的。实验室估计的N2O生产显着相关,在10个月期间在两个森林流域原位N2O扩散测量。硝化作用产生的一氧化二氮是最重要的排水良好的干扰流域,环境NO3-是高的网站。相反,反硝化N2O是最重要的排水不良网站附近的流相同的分水岭。反硝化N2O和充水孔隙之间的相关性和硝化N2O和环境NO3-之间的N2O生产硝化和反硝化之间的区别得到证实。该技术允许环境参数的定性研究,通过反硝化和硝化调节气态氮的损失。
The source of N2O produced in soil is often uncertain because denitrification and nitrification can occur simultaneously in the same soil aggregate. A technique which exploits the differential sensitivity of these processes to C2H2 inhibition is proposed for distinguishing among gaseous N losses from soils. Denitrification N2O was estimated from 24-h laboratory incubations in which nitrification was inhibited by 10-Pa C2H2. Nitrification N2O was estimated from the difference between N2O production under no C2H2 and that determined for denitrification. Denitrification N2 was estimated from the difference between N2O production under 10-kPa C2H2 and that under 10 Pa. Laboratory estimates of N2O production were significantly correlated with in situ N2O diffusion measurements made during a 10-month period in two forested watersheds. Nitrous oxide production from nitrification was most important on well-drained sites of a disturbed watershed where ambient NO3- was high. In contrast, denitrification N2O was most important on poorly drained sites near the stream of the same watershed. Distinction between N2O production from nitrification and denitrification was corroborated by correlations between denitrification N2O and water-filled pore space and between nitrification N2O and ambient NO3-. This technique permits qualitative study of environmental parameters that regulate gaseous N losses via denitrification and nitrification.