HLA membrane antigens: sequencing by intrinsic radioactivity.
HLA membrane antigens: sequencing by intrinsic radioactivity.
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HLA 膜抗原：通过内在放射性进行测序。
DOI：
10.1073/pnas.73.12.4487
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发表时间：
1976
影响因子：
11.1
通讯作者：
O. Smithies
中科院分区：
文献类型：
--
作者：
B. Ballou;D. McKean;E. Freedlender;O. Smithies
本文报道了从人淋巴母细胞系分离的混合抗原A1和B8的氨基末端的放射化学序列数据。在小规模组织培养中，用~(14)C标记的氨基酸和[~(14)C]丙酮酸进行细胞内标记。获得的比活度足够高和均匀，可以直接对抗原进行放射化学测序。用固相抗β2-微球蛋白免疫吸附剂吸附，再经电泳法分离出人类白细胞抗原。该方法应普遍适用于对分子的所有部分进行测序。
Radiochemical sequence data are presented for the amino termini of mixed HLA antigens A1 and B8 isolated from a human lymphoblastoid cell line. Cells were labeled intrinsically in small-scale tissue culture with 14C-labeled amino acids and [14C]pyruvate. The specific activities obtained were sufficiently high and uniform to permit direct radiochemical sequencing of the antigens. HLA antigens were isolated by adsorption to a solid-phase anti-beta2-microglobulin immunoadsorbent followed by electrophoresis. The method should be generally applicable and useful for sequencing all parts of the molecule.