Role of nicotinamide adenine dinucleotide as an effector in formation and reactions of acylglyceraldehyde-3-phosphate dehydrogenase.
Role of nicotinamide adenine dinucleotide as an effector in formation and reactions of acylglyceraldehyde-3-phosphate dehydrogenase.
复制标题
烟酰胺腺嘌呤二核苷酸作为效应物在酰基甘油醛-3-磷酸脱氢酶的形成和反应中的作用。
DOI：
10.1021/bi00522a028
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Bernhard,SA
中科院分区：
文献类型：
--
作者：
Malhotra,OP;Bernhard,SA
O. P. Malhotra* 和Sidney A. Bernhard* 摘要：发色酰基甘油醛-3-磷酸脱氢酶（FA-GPDH）的平衡光谱和反应特性先前已被报道。本文报道了这些性能的瞬态研究。与真正的3-磷酸甘油酰-酶一样，这些性质取决于结合的辅酶（NAD+）的存在。酰基酶对受体（磷酸盐和砷酸盐）的反应性与其NAD+诱导的光谱变化的程度平行[Malhotra，O. P.，& Bernhard，S. A.等人（1973）Proc. Acad. Sci USA 70，2077-2081]。用NAD”1”预孵育的FA-GPDH的瞬时脱酰是动力学双相的。快相对慢相的相对振幅取决于NAD+浓度，但与酰基受体的性质和浓度无关。在饱和的NAD”1”和受体浓度下，动力学二相性持续存在。NAD”1”对酰基-脱辅基酶谱的扰动也是ki-
O. P. Malhotra* and Sidney A. Bernhard* abstract: The equilibrium spectral and reactivity properties of a chromophoric acylglyceraldehyde-3-phosphate dehydrogenase (FA-GPDH) have been previously reported. Transient studies of these properties are reported herein. As with true 3-phosphoglyceroyl-enzyme these properties depend on the presence of bound coenzyme (NAD+). The reactivity of the acyl-enzyme toward acceptors (phosphate and arsenate) parallels the extent of its NAD+-induced spectral change [Malhotra, O. P., & Bernhard, S. A.(1973) Proc. Natl. Acad. Sci USA 70, 2077-2081]. The transcient deacylation of FA-GPDH, preincubated with NAD" 1", is kinetically biphasic. The relative amplitudes of the fast vs. the slowphase depend on NAD" 1" concentration but are independent of the nature and concentration of the acyl acceptor. At saturating NAD" 1" and acceptor concentrations, kinetic biphasicity persists. Perturbation of the acyl-apoenzyme spectrum by NAD" 1" is also ki-