MOLECULAR-CLONING OF PROTEIN 4.1, A MAJOR STRUCTURAL ELEMENT OF THE HUMAN-ERYTHROCYTE MEMBRANE SKELETON
MOLECULAR-CLONING OF PROTEIN 4.1, A MAJOR STRUCTURAL ELEMENT OF THE HUMAN-ERYTHROCYTE MEMBRANE SKELETON
复制标题
DOI：
10.1073/pnas.83.24.9512
复制
发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
MOHANDAS, N
中科院分区：
文献类型：
--
作者：
CONBOY, J;KAN, YW;MOHANDAS, N
蛋白4.1是一种重要的结构蛋白，在红系和多种非红系组织中表达。在哺乳动物红细胞中，它通过稳定血影蛋白-肌动蛋白相互作用在调节膜的机械稳定性和变形性的物理性质中起关键作用。本文报道了人红细胞蛋白4.1cDNA的分子克隆和鉴定，并从该核苷酸序列中获得了该蛋白的完整氨基酸序列。从克隆的红细胞蛋白4.1 cDNA制备的探针与来自多种非红细胞组织（包括脑、肝、胎盘、胰腺和肠）的不同mRNA种类杂交，这意味着红细胞和非红细胞蛋白4.1之间具有实质性同源性。克隆的红细胞蛋白4.1 cDNA的可用性应有利于研究这种蛋白质在红系细胞以及非红系细胞中的功能特性。
Protein 4.1 is an important structural protein that is expressed in erythroid and in a variety of non-erythroid tissues. In mammalian erythrocytes, it plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. We report here the molecular cloning and characterization of human erythrocyte protein 4.1 cDNA and the complete amino acid sequence of the protein derived from the nucleotide sequence. Probes prepared from the cloned erythrocyte protein 4.1 cDNA hybridized with distinct mRNA species from a wide variety of non-erythroid tissues, including brain, liver, placenta, pancreas, and intestine, implying substantial homology between erythroid and non-erythroid protein 4.1. The availability of cloned erythrocyte protein 4.1 cDNA should facilitate the study of the functional characteristics of this protein in erythroid as well as non-erythroid cells.