BRUSH BORDER PARTICULATES OF RENAL TISSUE
BRUSH BORDER PARTICULATES OF RENAL TISSUE
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DOI：
10.1126/science.162.3857.1009
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发表时间：
1968-01-01
期刊：
影响因子：
56.9
通讯作者：
WUNDRAM, IJ
中科院分区：
文献类型：
--
作者：
BINKLEY, F;KING, N;WUNDRAM, IJ
通过区域离心从匀浆和核糖体制剂中分离出含有肾组织大部分碱性磷酸酶活性的颗粒。这些颗粒具有高含量的磷脂和胆固醇，但用 1% 的脱氧胆酸盐处理并不能去除这些物质。与颗粒一起浓缩的酶活性是碱性磷酸酶、抗蛋白水解的肽酶、葡萄糖-6-磷酸酶、无机焦磷酸酶和三磷酸腺苷酶。颗粒积累亮氨酸，不受可溶性因子的刺激，并受到其他氨基酸的抑制；三磷酸腺苷刺激积累，并且不受嘌呤霉素抑制。这些颗粒似乎源自肾小管细胞刷状缘的膜。
Particulates containing a large part of the alkaline phosphatase activity of renal tissue were separated from homogenates and from ribosomal preparations by zonal centrifugation. The particles had a high content of phospholipid and cholesterol that was not removed by treatment with 1 percent deoxycholate. Enzymatic activities concentrated with the particles were the alkaline phosphatase, a peptidase resistant to proteolysis, glucose-6-phosphatase, inorganic pyrophosphatase, and adenosine triphosphatase. The particles accumulated leucine with no stimulation from soluble factors and with inhibition by other amino acids; the accumulation was stimulated by adenosine triphosphate and was not inhibited by puromycin. The particles appear to be derived from the membranes of the brush borders of tubular cells.