Phase-resolved magnetomotive OCT for imaging nanomolar concentrations of magnetic nanoparticles in tissues
Phase-resolved magnetomotive OCT for imaging nanomolar concentrations of magnetic nanoparticles in tissues
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DOI：
10.1364/oe.16.011525
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发表时间：
2008-07-21
期刊：
影响因子：
3.8
通讯作者：
Boppart, Stephen A.
中科院分区：
文献类型：
--
作者：
Oldenburg, Amy L.;Crecea, Vasilica;Boppart, Stephen A.
磁性纳米粒子（MNP）在磁共振和生物医学光学成像中变得越来越重要。我们描述了一种通过使用相位分辨谱域光学相干断层扫描（OCT）系统检测纳米级位移来对 MNP 进行成像的方法。生物组织和模型暴露在 56 和 100 Hz 调制的类似于 800 G 的磁场中，以机械方式驱动嵌入的氧化铁 MNP（直径类似于 20 nm）。通过过滤抗磁振动中的顺磁，可以实现组织模型内 27 μg/g（类似于 2 nM）MNP 的灵敏度。我们通过对局部应用的 MNP 在 2 小时内扩散到切除的大鼠肿瘤中的过程进行成像来证明生物学可行性。 (c) 2008 年美国光学学会。
Magnetic nanoparticles (MNPs) are increasingly important in magnetic resonance and biomedical optical imaging. We describe a method for imaging MNPs by detecting nanoscale displacements using a phase-resolved spectral-domain optical coherence tomography (OCT) system. Biological tissues and phantoms are exposed to similar to 800 G magnetic fields modulated at 56 and 100 Hz to mechanically actuate embedded iron oxide MNPs(similar to 20 nm diameter). Sensitivity to 27 mu g/g (similar to 2 nM) MNPs within tissue phantoms is achieved by filtering paramagnetic from diamagnetic vibrations. We demonstrate biological feasibility by imaging topically applied MNPs during their diffusion into an excised rat tumor over a 2 hour time period. (c) 2008 Optical Society of America.