Isolation of a deet-insensitive mutant of Drosophila melanogaster (Diptera: Drosophilidae)
Isolation of a deet-insensitive mutant of Drosophila melanogaster (Diptera: Drosophilidae)
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DOI：
10.1603/0022-0493-94.6.1584
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发表时间：
2001-12-01
影响因子：
2.2
通讯作者：
Saunders, CW
中科院分区：
文献类型：
--
作者：
Reeder, NL;Ganz, PJ;Saunders, CW
尽管N，N，-二乙基，1-3-甲基苯甲酰胺（dect）在驱虫产品中广泛使用，但对避蚊胺的驱虫性的分子基础一无所知。我们启动了一项分子遗传学计划，以阐明果蝇（Meigen）的dect排斥性的分子机制。避蚊胺的排斥性显然是由于空气中的蒸汽，因为野生型苍蝇在没有物理接触和黑暗中被避蚊胺处理过的表面所排斥。采用化学诱变和选择试验分离突变体。在一项选择试验中，突变果蝇进入了82 +/- 1%的含避蚊胺的试管。而野生型苍蝇仅进入6 +/-2%的含避蚊胺的管中，突变体被其它驱避剂苯甲醛和香茅醛驱避。该突变为隐性突变，位于X染色体上。
Despite the widespread use of N,N,-diethyl,1-3-methylbenzamide (dect) in insect repellent products, nothing is known about the molecular basis for the repellency of deet. We initiated a molecular genetics program to elucidate the molecular mechanism of dect repellency in Drosophila melanogaster (Meigen). Deet repellency was apparently due to airborne vapors, as wild type flies were repelled by a deet-treated surface in the absence of physical contact and in the dark. Mutant was isolated using chemical mutagenesis and a choice assay. In a choice assay, mutant flies entered 82 +/- 1% of deet-containing tubes. whereas wild type flies entered only 6 +/- 2% of deet-Containing tubes, The mutant was repelled by other repellents, benzaldehyde and citronellal. The mutation was recessive and located on the X chromosome.