Depressed production of beta-defensins from mouse splenic dendritic cells following thermal injury and its influence on susceptibility to infection.
Depressed production of beta-defensins from mouse splenic dendritic cells following thermal injury and its influence on susceptibility to infection.
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热损伤后小鼠脾树突状细胞β-防御素的产生减少及其对感染易感性的影响。
DOI：
10.1007/s00540-014-1882-y
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发表时间：
2015
期刊：
影响因子：
2.8
通讯作者：
Suzuki F.
中科院分区：
文献类型：
--
作者：
Kawasaki T.;Kawasaki C.;Sata T.;Kobayashi M.;Suzuki F.
目的β-防御素（BD）和树突状细胞（DC）是宿主抗微生物天然免疫的主要效应者。方法从小鼠脾脏分离DC，取1 × 106 cells/ml，加入LPS或SAC培养，用MTT法检测DC的增殖能力。测定培养后24小时收获的培养液的BD 1和BD 3以及抗菌活性（菌落计数，铜绿假单胞菌）。此外，树突状细胞进行了测试BD mRNA通过RT-PCR. Results 65%的细菌杀伤活性显示由正常小鼠的脾DC的培养液，而只有15%的杀伤活性显示由FT烧伤小鼠的脾DC的培养液。X射线照射的NOD SCID IL-2 r γnullmice接种来自FT烧伤小鼠的脾DC显示对P的敏感性增加。与正常小鼠相比，小鼠脾DC组成性表达BD 1 mRNA，刺激后表达BD 3 mRNA。这些BD由小鼠脾DC产生。与正常小鼠的DC相比，FT烧伤小鼠的DC产生的BD 1和BD 3在其culture fluids.ConclusionsThese结果表明，（1）DC从小鼠脾脏有能力产生BD，和（2）BD的DC的生产受到强烈的热损伤应激的影响。由于FT烧伤小鼠对P.铜绿假单胞菌感染时，DC产生的BD可能在宿主的抗菌药物耐药性中发挥重要作用。
PurposeBeta-defensins (BDs) and dendritic cells (DC) have been described as major effectors on host antimicrobial innate immunities. In the present study, the ability of DC to produce BDs was explored using DC from normal mice and full-thickness (FT)-burned mice.MethodsDCs were isolated from spleens of mice, and 1 × 106cells/ml of them were cultured with LPS or SAC. Culture fluids harvested 24 h after cultivation were assayed for BD1 and BD3 and antibacterial activity (colony-counting,Pseudomonas aeruginosa). Also, DCs were tested for BD mRNAs by RT-PCR.ResultsSixty-five percent of the bacterial killing activity was shown by the culture fluids of splenic DC from normal mice, while only 15 % killing activity was shown by the culture fluids of splenic DC from FT-burned mice. X-irradiated NOD SCID IL-2rγnullmice inoculated with splenic DC from FT-burned mice showed increased susceptibility toP. aeruginosainfection compared to those from normal mice. Mice splenic DC expressed BD1 mRNA constitutively and expressed BD3 mRNA after stimulation. These BDs were produced by mice splenic DC. As compared with DC from normal mice, DC from FT-burned mice produced decreased amounts of BD1 and BD3 in their culture fluids.ConclusionsThese results indicate that (1) DC from spleens of mice have an ability to produce BDs, and (2) the production of BDs by DC is influenced strongly by thermally injured stress. Since FT-burned mice are susceptible toP. aeruginosainfection, BDs produced by DC may play an important role on the host’s antibacterial resistance.