Selection for high-level resistance by chronic triclosan exposure is not universal
Selection for high-level resistance by chronic triclosan exposure is not universal
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DOI：
10.1093/jac/dkh168
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发表时间：
2004-05-01
影响因子：
5.2
通讯作者：
Gilbert, P
中科院分区：
文献类型：
--
作者：
McBain, AJ;Ledder, RG;Gilbert, P
目的：探讨三氯生暴露对重要牙科细菌抗菌敏感性的影响。方法：采用梯度平板技术暴露具核梭杆菌、鼠李糖乳杆菌、亚黄奈瑟菌、牙龈卟啉单胞菌、内氏放线菌、黑普氏菌、口腔链球菌、血链球菌、变形链球菌和韦荣球菌反复分解，使三氯生浓度不断升高，达到亚致死浓度。大肠杆菌ATCC 8739作为显示三氯生抗性发展特征的生物体被包括在内。在接触杀菌剂之前和之后测定了对氯己定、甲硝唑和四环素的 MIC 值。结果：N. subflava、Pr.黑质 Po.牙龈杆菌和大肠杆菌对三氯生高度敏感（MIC 范围 0.1-3.9 mg/L），而乳酸杆菌和变形链球菌则不太敏感（MIC 范围 15.6-20.8 mg/L）。三氯生暴露导致阳性对照大肠杆菌的三氯生敏感性显着降低（大约 400 倍）（P < 0.01），尽管其对洗必泰、甲硝唑和四环素的 MIC 没有显着改变。 Pr 的三氯生敏感性 (MIC) 出现轻微（类似于两倍）下降。 nigrescens 以及 S. sanguis 和 S.oralis (MBC)。 Mean changes in susceptibilities (MIC and MBC) of the oral species to chlorhexidine, metronidazole and tetracycline did not exceed two-fold, although chlorhexidine MBCs for S. sanguis were markedly, but transiently, increased.Conclusions: These data fail to demonstrate biologically significant drug resistance in triclosan-exposed bacteria and suggest that markedly decreased triclosan susceptibility, although confirmed for E. coli, is not a universal现象。其他细菌可能拥有比 FabI 更敏感的靶点，这些靶点高度保守，可能控制三氯生活性。
Objectives: To investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria.Methods: A gradient plate technique was used to expose Fusobacterium nucleatum, Lactobacillus rhamnosus, Neisseria subflava, Porphyromonas gingivalis, Actinomyces naeslundii, Prevotella nigrescens, Streptococcus oralis, Streptococcus sanguis, Streptococcus mutans and Veillonella dispar repeatedly to escalating, sublethal concentrations of triclosan. Escherichia coli ATCC 8739 was included as an organism showing the triclosan resistance development trait. MIC values towards chlorhexidine, metronidazole and tetracycline were determined before and after biocide exposure.Results: N. subflava, Pr. nigrescens Po. gingivalis and E. coli were highly susceptible to triclosan (MIC range 0.1-3.9 mg/L), whereas the lactobacillus and S. mutans were less susceptible (MIC range 15.6-20.8 mg/L). Triclosan exposure resulted in a highly significant (similar to400-fold) reduction in triclosan susceptibility (P < 0.01) for the positive control E. coli, although its MICs towards chlorhexidine, metronidazole and tetracycline were not significantly altered. Minor (similar totwo-fold) decreases in triclosan susceptibility (MIC) occurred for Pr. nigrescens and in S. sanguis and S. oralis (MBC). Mean changes in susceptibilities (MIC and MBC) of the oral species to chlorhexidine, metronidazole and tetracycline did not exceed two-fold, although chlorhexidine MBCs for S. sanguis were markedly, but transiently, increased.Conclusions: These data fail to demonstrate biologically significant drug resistance in triclosan-exposed bacteria and suggest that markedly decreased triclosan susceptibility, although confirmed for E. coli, is not a universal phenomenon. Other bacteria possibly possess more susceptible targets than FabI that are highly conserved, which may govern triclosan activity.