Phase-Contrast OCT Imaging of Transverse Flows in the Mouse Retina and Choroid
Phase-Contrast OCT Imaging of Transverse Flows in the Mouse Retina and Choroid
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DOI：
10.1167/iovs.07-1627
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发表时间：
2008-11-01
影响因子：
4.4
通讯作者：
Fraser, Scott E.
中科院分区：
文献类型：
--
作者：
Fingler, Jeff;Readhead, Carol;Fraser, Scott E.
目的.为了验证一种新的相位对比光学相干断层扫描（OCT）系统可以成像活体小鼠视网膜和脉络膜血管的假设。开发了一种用于小鼠视网膜成像的高速谱域光学相干断层扫描（SDOCT）系统，该系统在每次轴向扫描（A扫描）时测量视网膜整个深度的反射。在横跨小鼠视网膜的横向线上采集多个A扫描提供了二维横截面图像（B扫描）;几个相邻的B扫描可以组装成三维OCT图像。为了使视网膜血管中的流动性和横向流动可视化，从针对相同视网膜横截面连续采集的多个B扫描计算每个位置的相位的统计方差。这种相位变化的测量提供了一个直接测量的运动在一个大的动态范围内的流动速度。使用通过视网膜的多个二维横截面图像切片来创建活体小鼠视网膜的三维相衬图像。对于这里提供的数据，每个横截面相衬切片分别来自于在25 ms或50 ms内采集的100或200个横向像素的五个图像。该方法提供了清晰的识别运动区域在不同的深度，包括在视网膜微血管和脉络膜血管的流量。相位对比OCT能够实现体内视网膜和脉络膜血管的三维可视化。（Invest Ophthalmol维斯科学。2008;49：5055-5059）DOI：10.1167/iovs.07-1627
PURPOSE. To test the hypothesis that a novel phase-contrast optical coherence tomography (OCT) system can image retinal and choroidal vessels in the living mouse.METHODS. A high-speed spectral domain optical coherence tomography (SDOCT) system, which measures the reflections for the entire depth of the retina at once with each axial scan (A-scan), was developed for mouse retinal imaging. Acquiring multiple A-scans over a transverse line across the mouse retina offers a two-dimensional cross-sectional image (B-scan); several neighboring B-scans can be assembled into a three-dimensional OCT image. To visualize mobility and transverse flow in retinal vessels, the statistical variance of phase for each location was calculated from multiple B-scans acquired successively for the same retinal cross-section. Such measures of phase variance offer a direct measure of motions over a large dynamic range of flow velocities.RESULTS. Three-dimensional phase-contrast images of the live mouse retina were created using multiple two-dimensional cross-sectional image slices through the retina. For the data presented here, each cross-sectional phase-contrast slice resulted from five images of 100 or 200 transverse pixels, acquired over 25 ms or 50 ms, respectively. The approach offered clear identification of motion regions at different depths, including flow in the retinal microvasculature and in the choroidal vessels.CONCLUSIONS. Phase-contrast OCT enables three-dimensional visualization of retinal and choroidal vasculature in vivo. (Invest Ophthalmol Vis Sci. 2008;49:5055-5059) DOI:10.1167/iovs.07-1627