OsKASI, a β-ketoacyl-[acyl carrier protein] synthase I, is involved in root development in rice (Oryza sativa L.)
OsKASI, a β-ketoacyl-[acyl carrier protein] synthase I, is involved in root development in rice (Oryza sativa L.)
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OsKASI 是一种 β-酮酰基-[酰基载体蛋白]合酶 I，参与水稻 (Oryza sativa L.) 的根部发育
DOI：
10.1007/s00425-015-2296-2
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发表时间：
2015-07-01
期刊：
影响因子：
4.3
通讯作者：
Zhu, Shihua
中科院分区：
文献类型：
--
作者：
Ding, Wona;Lin, Li;Zhu, Shihua
OsKASI参与FA合成在水稻根系发育中起关键作用。根系在植物养分和水分获取中起着重要作用。然而，水稻根系发育和分子调控的机制尚不清楚。在这里，我们描述了一个水稻（Oryza sativa L.）突变体，由于细胞伸长缺陷而导致根缩短。基于图谱的克隆结果表明，该突变发生在拟南芥β -酮酰基-[酰基载体蛋白]合成酶I （KASI）的同源物3-氧酰基合成酶上，因此被命名为OsKASI。研究发现，OsKASI蛋白在植物各组织中普遍表达，并定位于质体中。此外，OsKASI缺乏症还导致生育力下降，根系和种子中脂肪酸组成和含量发生显著变化。我们的研究结果表明，OsKASI参与FA合成是水稻根系发育所必需的。
The involvement of OsKASI in FA synthesis is found to play a critical role in root development of rice.The root system plays important roles in plant nutrient and water acquisition. However, mechanisms of root development and molecular regulation in rice are still poorly understood. Here, we characterized a rice (Oryza sativa L.) mutant with shortened roots due to a defect in cell elongation. Map-based cloning revealed that the mutation occurred in a putative 3-oxoacyl-synthase, an ortholog of beta-ketoacyl-[acyl carrier protein] synthase I (KASI) in Arabidopsis, thus designated as OsKASI. OsKASI was found to be ubiquitously expressed in various tissues throughout the plant and OsKASI protein was localized in the plastid. In addition, OsKASI deficiency resulted in reduced fertility and a remarkable change in fatty acid (FA) composition and contents in roots and seeds. Our results demonstrate that involvement of OsKASI in FA synthesis is required for root development in rice.