Involvement of Spinal Neurotrophin-3 in Electroacupuncture Analgesia and Inhibition of Spinal Glial Activation in Rat Model of Monoarthritis
Involvement of Spinal Neurotrophin-3 in Electroacupuncture Analgesia and Inhibition of Spinal Glial Activation in Rat Model of Monoarthritis
复制标题
脊髓神经营养素3参与电针镇痛及抑制单关节炎大鼠模型脊髓胶质细胞活化
DOI：
10.1016/j.jpain.2011.03.002
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发表时间：
2011-09-01
期刊：
影响因子：
4
通讯作者：
Wu, Gen-Cheng
中科院分区：
文献类型：
--
作者：
Mi, Wen-Li;Mao-Ying, Qi-Liang;Wu, Gen-Cheng
虽然电针（EA）已被证明可以有效地缓解关节炎相关疼痛，但其镇痛机制仍需进一步研究。在这里，脊髓神经营养因子-3（NT-3）参与EA对完全弗氏佐剂（CFA）诱导的炎性疼痛的镇痛作用进行了检查。本研究结果表明：1）重复电针同侧GB 30和GB 34穴可显著抑制CFA诱发的痛敏，2）电针可显著增强CFA诱发的脊髓NT-3 mRNA和蛋白水平的上调，3）在电针治疗期间鞘内注射NT-3特异性反义寡核苷酸（ODN）7天可显著减弱电针镇痛作用。电针对脊髓胶质细胞标志物GFAP、小胶质细胞标志物OX-42以及促炎细胞因子IL-1 β、IL-6和TNF-α表达的抑制作用在NT-3反义ODN递送后显著减弱。这些结果表明，内源性NT-3可能参与EA对大鼠炎性疼痛的镇痛作用，通过抑制脊髓胶质细胞活性以及促炎细胞因子的产生来介导。本研究为探讨NT-3/glia/cells在神经胶质细胞凋亡中的作用提供了新的思路。本研究旨在探讨细胞因子在针灸治疗关节炎疼痛中的作用，并为针灸治疗关节炎疼痛的机制提供新的思路。(C)2011年美国疼痛协会American Pain Society
Although electroacupuncture (EA) has been proven to effectively relieve pain associated with arthritis, the underlying mechanism of EA analgesia requires further investigation. Here, the involvement of spinal neurotrophin-3 (NT-3) in EA's analgesic effects on complete Freund's adjuvant (CFA)-induced inflammatory pain was examined. The present study demonstrated that: 1) repeated EA stimulation of ipsilateral GB30 and GB34 acupoints remarkably suppressed CFA-induced hyperalgesia; 2) EA treatment markedly enhanced the upregulation of spinal NT-3 mRNA and protein levels following CFA injection; 3) antisense oligodeoxynucleotides (ODN) specifically against NT-3 intrathecally administered during EA treatment for 7 days significantly attenuated the EA analgesia; and 4) the suppressed expression of spinal GFAP (astrocytic marker), OX-42 (microglial marker) as well as proinflammatory cytokines, interleukin (IL)-1 beta, IL-6 and tumor necrosis factor (TNF)-alpha by EA treatment was significantly attenuated following NT-3 antisense ODN delivery. These results suggested that endogenous NT-3 may be involved in the analgesic effect of EA on inflammatory pain in rats, mediated through the inhibition of spinal glial activity as well as proinflammatory cytokine production.Perspective: The present study may initiate a discussion on the possible roles of NT-3/glia/cytokines in the therapeutic effects of acupuncture and provide insight on the mechanism underlie the analgesic effects of acupuncture on pain associated with arthritis. (C) 2011 by the American Pain Society