Widespread RNA 3′-end oligouridylation in mammals
Widespread RNA 3′-end oligouridylation in mammals
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DOI：
10.1261/rna.029306.111
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发表时间：
2012-03-01
期刊：
影响因子：
4.5
通讯作者：
McManus, Michael T.
中科院分区：
文献类型：
--
作者：
Choi, Yun S.;Patena, Weronika;McManus, Michael T.
RNA的非模板化3'-末端寡核苷酸化发生在许多物种中，包括人类。与熟悉的多聚腺苷酸化现象不同，在高等真核生物中，尿苷非模板化添加到RNA中的特征很差。最近的研究报道了小RNA和mRNA的非模板化3 '端寡核苷酸化。寡核苷酸化参与从成熟物种的生物发生到周转的microRNA生物学的许多方面，并且它还可以通过促进保护性5 '帽结构的脱帽来标记长mRNA以进行降解。为了确定寡核苷酸化在高等真核生物中的普遍性，我们使用下一代测序技术深入研究了人类细胞中的小RNA群体。我们的数据显示，在许多种类的RNA的3 '端广泛存在非模板核苷酸，其中短的尿苷是最常见的核苷酸。
Nontemplated 3'-end oligouridylation of RNA occurs in many species, including humans. Unlike the familiar phenomenon of polyadenylation, nontemplated addition of uridines to RNA is poorly characterized in higher eukaryotes. Recent studies have reported nontemplated 3'-end oligouridylation of small RNAs and mRNAs. Oligouridylation is involved in many aspects of microRNA biology from biogenesis to turnover of the mature species, and it may also mark long mRNAs for degradation by promoting decapping of the protective 5'-cap structure. To determine the prevalence of oligouridylation in higher eukaryotes, we used next-generation sequencing technology to deeply examine the population of small RNAs in human cells. Our data revealed widespread nontemplated nucleotide addition to the 3' ends of many classes of RNA, with short stretches of uridine being the most frequently added nucleotide.