A regulatory gene induces trichome formation and embryo lethality in tomato
A regulatory gene induces trichome formation and embryo lethality in tomato
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调控基因诱导番茄毛状体形成和胚胎致死
DOI：
10.1073/pnas.1100532108
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发表时间：
2011-07-19
影响因子：
11.1
通讯作者：
Ye, Zhibiao
中科院分区：
文献类型：
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作者：
Yang, Changxian;Li, Hanxia;Ye, Zhibiao
毛状体是起源于气生表皮的普遍生物结构，是研究植物细胞分化的良好模型。虽然在Rosids中调节毛状体形成的途径已经被很好地表征，但直到最近才在Asterids中发现了一些与毛状体形成有关的基因。在这项研究中，我们克隆了番茄毛状体形成所必需的Woolly （Wo）。转基因实验表明，毛状表型是由Wo突变引起的，该突变编码含有bZIP基序和START结构域的同源结构域蛋白。我们鉴定了Wo的三个等位基因，发现每个等位基因都含有一个错义突变，分别导致C端氨基酸替换。基因芯片和表达分析表明，b型细胞周期蛋白基因SlCycB2的表达可能受到Wo的调控，该基因也参与了毛状体的形成。通过RNAi抑制Wo或SlCycB2的表达减少了I型毛的数量，并且在它们之间检测到直接的蛋白-蛋白相互作用，这意味着这两种蛋白可能共同调节这种类型的毛的形成。发育中的种子细胞学观察和Wo转录本分析表明，胚发育也与Wo有关。
Trichomes are universal biological structures originating from the aerial epidermis, which serve as an excellent model to study plant differentiation at the cell level. Although the pathway regulating trichome formation in the Rosids has been well characterized, only very recently a few genes were identified for trichome initiation in the Asterids. In this study, we cloned Woolly (Wo), essential for trichome formation in tomato. Transgenic experiments revealed that the woolly phenotype is caused by the mutation in Wo which encodes a homeodomain protein containing a bZIP motif and a START domain. We identified three alleles of Wo and found that each allele contains a missense mutation, which respectively results in an amino acid substitution at the C terminus. Microarray and expression analysis showed that the expression of a B-type cyclin gene, SlCycB2, is possibly regulated by Wo, which also participates in trichome formation. Suppression of Wo or SlCycB2 expression by RNAi decreased the number of type I trichomes, and direct protein–protein interaction was detected between them, implying that both proteins may work together in the regulation of this type of trichome formation. Cytological observation and Wo transcript analysis in the developing seeds showed that embryo development was also correlated with Wo.