Antimicrobial efficacy and wound-healing property of a topical ointment containing nitric-oxide-loaded zeolites
Antimicrobial efficacy and wound-healing property of a topical ointment containing nitric-oxide-loaded zeolites
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DOI：
10.1099/jmm.0.067322-0
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发表时间：
2014-02-01
影响因子：
3
通讯作者：
Joshi, Suresh G.
中科院分区：
文献类型：
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作者：
Neidrauer, Michael;Ercan, Utku K.;Joshi, Suresh G.
通过创面敷料局部输送一氧化氮(NO)有可能减少伤口感染，促进急慢性伤口的愈合。这项研究表征了一种软膏的抗菌效果，该软膏含有无负载、锌交换的A沸石，与水接触时会释放NO。用化学发光检测系统测定软膏中NO的释放率。最低杀菌浓度测定使用五种常见伤口病原体，包括革兰氏阴性菌(大肠杆菌和鲍曼不动杆菌)、革兰氏阳性菌(表皮葡萄球菌和耐甲氧西林金黄色葡萄球菌)和真菌(白色念珠菌)。在软膏作用1-8h后的4个时间点进行对数减数实验，以表征抗菌活性的时间依赖性。用培养的3T3成纤维细胞评价软膏24 h后的细胞毒性。细菌的最低杀菌浓度(MMC)(5x10(7)c.f.u)对白色念珠菌的最低抑菌浓度为5×10(4)c.f.u。为50 mg软膏(ml介质)(-1)。与未经处理的生物相比，接触无沸石软膏8小时后，观察到细菌活力减少了5到8个对数，真菌活力减少了3个对数。成纤维细胞在与抗菌试验中使用的相同浓度的无沸石软膏中暴露24小时后仍然存活。在平行的研究中，Zucker肥胖大鼠的全层皮肤伤口比对照软膏治疗的伤口愈合得更快。这些数据表明，含有非负载沸石的软膏可能被用作广谱抗菌伤口愈合敷料。
Topical delivery of nitric oxide (NO) through a wound dressing has the potential to reduce wound infections and improve healing of acute and chronic wounds. This study characterized the antibacterial efficacy of an ointment containing NO-loaded, zinc-exchanged zeolite A that releases NO upon contact with water. The release rate of NO from the ointment was measured using a chemiluminescence detection system. Minimum bactericidal concentration assays were performed using five common wound pathogens, including Gram-negative bacteria (Escherichia coli and Acinetobacter baumannii), Gram-positive bacteria (Staphylococcus epidermidis and meticillin-resistant Staphylococcus aureus) and a fungus (Candida albicans). The time dependence of antimicrobial activity was characterized by performing log-reduction assays at four time points after 1-8 h ointment exposure. The cytotoxicity of the ointment after 24 h was assessed using cultured 3T3 fibroblast cells. Minimum microbicidal concentrations (MMCs) for bacterial organisms (5x10(7) c.f.u.) ranged from 50 to 100 mg ointment (ml media)(-1); the MMC for C. albicans (5x10(4) c.f.u.) was 50 mg ointment (ml media)(-1). Five to eight log reductions in bacterial viability and three log reductions in fungal viability were observed after 8 h exposure to NO zeolite ointment compared with untreated organisms. Fibroblasts remained viable after 24 h exposure to the same concentration of NO zeolite ointment as was used in antimicrobial tests. In parallel studies, full-thickness cutaneous wounds on Zucker obese rats healed faster than wounds treated with a control ointment. These data indicate that ointment containing NO-loaded zeolites could potentially be used as a broad-spectrum antimicrobial wound-healing dressing.