Formalin-inactivated bovine RSV vaccine influences antibody levels in bronchoalveolar lavage fluid and disease outcome in experimentally infected calves
Formalin-inactivated bovine RSV vaccine influences antibody levels in bronchoalveolar lavage fluid and disease outcome in experimentally infected calves
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DOI：
10.1016/j.vaccine.2005.04.032
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发表时间：
2005-08-31
期刊：
影响因子：
5.5
通讯作者：
Gershwin, LJ
中科院分区：
文献类型：
--
作者：
Kalina, WV;Woolums, AR;Gershwin, LJ
呼吸道合胞病毒（RSV）可引起小牛和人类婴儿严重的呼吸道疾病。为了应对疫情，开发了福尔马林灭活（FI）-RSV疫苗，发现在活RSV感染后会加剧疾病。我们在小牛中再现了疫苗接种诱导的疾病增强，并从两项研究中筛选了支气管肺泡灌洗液（BALF）中的各种抗体同种型：一项研究具有疾病增强，另一项研究具有中度保护作用，这是由Fl-牛RSV（BRSV）疫苗接种引起的。感染前，半保护性接种小牛产生BRSV特异性BALF IgG 1，但不产生伊加和IgG 2;而增强疾病小牛在BALE中未能产生BRSV特异性IgG 1。最终，RSV疫苗的配制和递送影响呼吸道分泌物中的保护性抗体水平。(c)2005爱思唯尔有限公司保留所有权利。
Respiratory syncytial virus (RSV) causes severe respiratory disease in calves and human infants. In response to outbreaks, formalin inactivated (FI)-RSV vaccines were developed and found to exacerbate disease following a live RSV infection. We have reproduced vaccination induced disease enhancement in calves and screened various antibody isotypes in bronchoalveolar lavage fluid (BALF) from two studies: one with disease enhancement and another where moderate protection resulted from Fl-bovine RSV (BRSV) vaccination. Semi-protected vaccinated calves produced BRSV-specific BALF IgG1 but not IgA and IgG2 prior to infection; whereas, calves with enhanced disease failed to develop BRSV-specific IgG1 in BALE Ultimately, the formulation and delivery of RSV vaccines influences protective antibody levels in respiratory secretions. (c) 2005 Elsevier Ltd. All rights reserved.